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Synthesis, Characterization and Fluorescence Properties of Zn(II) and Cu(II) Complexes: DNA Binding Study of Zn(II) Complex

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Abstract

Zinc(II) and copper(II) complexes containing Schiff base, 2- methoxy-6((E)-(phenylimino) methyl) phenol ligand (HL) were synthesized and characterized by elemental analysis, IR, NMR, and single crystal X-ray diffraction technique. The fluorescence properties and quantum yield of zinc complex were studied. Our data showed that Zn complex could bind to DNA grooves with Kb = 104 M−1. Moreover, Zn complex could successfully be used in staining of DNA following agarose gel electrophoresis. MTT assay showed that Zn complex was not cytotoxic in MCF-7 cell line. Here, we introduce a newly synthesized fluorescence probe that can be used for single and double stranded DNA detection in both solution and agarose gels.

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Acknowledgments

The Research Council of Ferdowsi University of Mashhad is acknowledged for financial support (Grant No. 3/26199). Thanks to Dr. Sattar Saberi for his constructive comments. The support of NSF-MRI Grant #1228232 for the purchase of the diffractometer is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran

    Parirokh Lavaee, Hossein Eshtiagh-Hosseini, Mohammad Reza Housaindokht & Abbas Ali Esmaeili

  2. Department of Chemistry, Tulane University, New Orleans, LA, 70118, USA

    Joel T. Mague

  3. Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Khalil Abnous

Authors
  1. Parirokh Lavaee
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  2. Hossein Eshtiagh-Hosseini
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  4. Joel T. Mague
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  5. Abbas Ali Esmaeili
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Correspondence to Hossein Eshtiagh-Hosseini.

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Lavaee, P., Eshtiagh-Hosseini, H., Housaindokht, M.R. et al. Synthesis, Characterization and Fluorescence Properties of Zn(II) and Cu(II) Complexes: DNA Binding Study of Zn(II) Complex. J Fluoresc 26, 333–344 (2016). https://doi.org/10.1007/s10895-015-1719-6

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  • DOI: https://doi.org/10.1007/s10895-015-1719-6

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