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DNA Folding Transition in Presence of Naphthylhydroxamic Acids as Revealed by Fluorescence Microscopic Single Molecule Observation Method

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Abstract

Fluorescence microscopy is a field of growing applications in various fields as it can be used to study immunofluorescence, fluorescent proteins techniques, live-cell imaging and many more. The present investigation is based on this single molecule observation technique to observe the effect of N-arylhydroxamic acids on λ plasmid DNA and linear Bacteriophage T7 DNA. The compounds under present investigation include N-1-naphthyl-o-methylbenzohydroxamic acid, N-1-naphthyl-p-methylbenzohydroxamic acid, N-1-naphthyl ethoxy benzo hydroxamic acid, N-1-naphthylphenylacetohydroxamic acid and N-1-naphthyl valero hydroxamic acid. The hydrodynamic radius, R H of λ DNA and long-axis length, l of T7 DNA were determined from the direct observation of Brownian motion of the DNA molecules in the absence and presence of hydroxamic acids. Folding transition was observed for λ DNA as well as T7 DNA in the presence of naphthyl hydroxamic acids. N-1-naphthylvalerohydroxamic acid was found to be most effective.

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Acknowledgments

Authors are grateful to the University Grants Commission, New Delhi for providing financial support under SAP program [grant number F – 540/9/DRS/2010].

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Authors and Affiliations

  1. School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, 492010, Chhattisgarh, India

    Priyanka Singh & Rama Pande

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  1. Priyanka Singh
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  2. Rama Pande
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Correspondence to Rama Pande.

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Singh, P., Pande, R. DNA Folding Transition in Presence of Naphthylhydroxamic Acids as Revealed by Fluorescence Microscopic Single Molecule Observation Method. J Fluoresc 26, 67–73 (2016). https://doi.org/10.1007/s10895-015-1661-7

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  • DOI: https://doi.org/10.1007/s10895-015-1661-7

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