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Pyridoxal Based Fluorescent Chemosensor for Detection of Copper(II) in Solution With Moderate Selectivity and Live Cell Imaging

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Abstract

A pyridoxal-based fluorescent probe HL was synthesized for the detection of Cu2+ in methanol with moderate selectivity. Upon addition of Cu2+, to the solution of the probe in methanol exhibited a remarkable change in emission at 500 nm. With the limit of detection of 10 μM, the probe could well meet the recommended (less than 32 μM in drinking water) of the World Health Organization (WHO). The intracellular Cu2+ imaging behaviour of HL was carried out on HeLa cells.

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Acknowledgments

Financial support from the University Grants Commission for senior research fellowship to S. Mandal [Sanction No. UGC/847/Jr. Fellow (Upgradation)] is gratefully acknowledged. SKM is grateful to DST-PURSE PROGRAMME for partial financial support of the biological work.

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Authors and Affiliations

  1. Department of Chemistry, University of Calcutta, 92, A. P. C. Road, Kolkata, 700009, India

    Senjuti Mandal & Sanchita Goswami

  2. Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, 741235, India

    Sushil Kumar Mandal & Anisur Rahman Khuda-Bukhsh

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  1. Senjuti Mandal
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Mandal, S., Mandal, S.K., Khuda-Bukhsh, A.R. et al. Pyridoxal Based Fluorescent Chemosensor for Detection of Copper(II) in Solution With Moderate Selectivity and Live Cell Imaging. J Fluoresc 25, 1437–1447 (2015). https://doi.org/10.1007/s10895-015-1634-x

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