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A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine

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Abstract

A new fluorescent probe (probe 1) was developed for recognition of cysteine (Cys) and homocysteine (Hcy). Probe 1 exhibited a large absorption peak blue-shift (107 nm) as well as enhanced fluorescence responses to Cys/Hcy based on cyclization of thiol containing amino acids with aldehydes, inhibiting the C = N isomerization-induced quenching process by an intramolecular hydrogen bond. The detection mechanism was proved by 1H NMR and mass spectrometry analysis.

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (21102037).

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Authors and Affiliations

  1. College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Puhui Xie, Guangqin Gao, Juanjuan Liu, Qiu Jin & Guoyu Yang

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  1. Puhui Xie
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  2. Guangqin Gao
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  3. Juanjuan Liu
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  4. Qiu Jin
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  5. Guoyu Yang
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Correspondence to Puhui Xie.

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Xie, P., Gao, G., Liu, J. et al. A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine. J Fluoresc 25, 1315–1321 (2015). https://doi.org/10.1007/s10895-015-1619-9

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  • DOI: https://doi.org/10.1007/s10895-015-1619-9

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