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Development of a Nile-Blue Based Chemodosimeter for Hg2+ in Aqueous Solution and its Application in Biological Imaging

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Abstract

A Nile blue-based chemodosimeter was newly synthesized. It can detect Hg2+ in aqueous solution based on desulfurization reaction. Upon its addition into aqueous Hg2+ ion solution, it exhibited a considerable blue-shift in its absorption and obvious fluorescence quenching. The detection mechanism was proved by mass spectrometry analysis and Gaussian calculations. Detection at an emission of 685 nm was extremely sensitive, with a detection limit of 2.5 × 10−9 mol/L. The fluorescent images in living cells and zebrafish demonstrate its potential for studying the accumulation of mercury species in organism.

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Acknowledgments

This work was supported by the Public science and technology research funds projects of ocean (201505021–2, 201005023–4), and Youth science funds project of ocean (2013560).

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Authors and Affiliations

  1. The Institute of Seawater Desalination and Multipurpose Utilization, SOA, Tianjin, China

    Mingming Hu, Jianhua Yin, Yahong Li & Xiaofang Zhao

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  1. Mingming Hu
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  2. Jianhua Yin
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  3. Yahong Li
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  4. Xiaofang Zhao
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Correspondence to Mingming Hu.

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Hu, M., Yin, J., Li, Y. et al. Development of a Nile-Blue Based Chemodosimeter for Hg2+ in Aqueous Solution and its Application in Biological Imaging. J Fluoresc 25, 403–408 (2015). https://doi.org/10.1007/s10895-015-1527-z

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  • DOI: https://doi.org/10.1007/s10895-015-1527-z

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