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Analysis of Cancer Marker in Tissues with Hadamard Transform Fluorescence Spectral Microscopic Imaging

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Abstract

Quantum dots (QDs) probes were used to tag and trace cancer biomarkers in cancer tissues based on the system of home-made Hadamard transform (HT) spectral microscopic imaging, which can be applied to provide high-resolution fluorescence spectrum and image of single cells and tissues. In situ fluorescence imaging for cancer marker proteins, such as estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), proliferating cell nuclear antigen (PCNA) and cytokeratin 20 (CK20) in tumor tissues, were realized by using the HT system to capture quantitative information for these proteins when tumor tissues were immunostained with QDs probes. A method to evaluate tumor malignancy of the specimens based on in situ analysis of distribution of marker proteins was proposed based on the comparative study of positive samples and negative controls. The investigation of ER contents of the cores in breast cancer tissue microarrays (TMAs) shows that the technique of QDs-immunohistochemistry (IHC)/HT spectral imaging is more sensitive than conventional IHC method. The results also demonstrate that the QDs-IHC/HT spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside tumor tissues, and the coupling of HT spectral imaging to the probing of subcellular molecules with QDs has great potential in biology and medical diagnosis.

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Acknowledgments

We gratefully acknowledge the support of the National Basic Research Program of China (973 Program, nos. 2011CB933600 and 2006CB933100), the Science Fund for Creative Research Groups of NSFC (nos. 20621502 and 20921062), the National Natural Science Foundation of China (21275110, 21005056), and the Fundamental Research Funds for the Central Universities (20100141110015).

Author information

Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China

    Hao Xu, Yue He, Hong-Wu Tang, Zhi-Ling Zhang & Dai-Wen Pang

  2. Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI) of the Chinese Academy of Sciences (CAS), Lanzhou, 730000, China

    Hao Xu

  3. Department of Oncology, Zhongnan Hospital of Wuhan University & Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan, 430071, China

    Chuang Chen & Yan Li

Authors
  1. Hao Xu
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  2. Chuang Chen
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  3. Yue He
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  4. Hong-Wu Tang
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  5. Zhi-Ling Zhang
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  6. Yan Li
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  7. Dai-Wen Pang
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Corresponding authors

Correspondence to Hao Xu or Hong-Wu Tang.

Additional information

This work was supported by the National Basic Research Program of China (973 Program, nos. 2011CB933600 and 2006CB933100), the Science Fund for Creative Research Groups of NSFC (nos. 20621502 and 20921062), the National Natural Science Foundation of China (21275110, 21005056), and the Fundamental Research Funds for the Central Universities (20100141110015).

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Xu, H., Chen, C., He, Y. et al. Analysis of Cancer Marker in Tissues with Hadamard Transform Fluorescence Spectral Microscopic Imaging. J Fluoresc 25, 397–402 (2015). https://doi.org/10.1007/s10895-015-1525-1

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  • DOI: https://doi.org/10.1007/s10895-015-1525-1

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