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Fluorescence Spectroscopy as a Tool for Determination of Coumarins by Multivariate Calibration

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Abstract

At present, it is necessary to check the quality of many food products in which the content of coumarins is limited. Since a rapid and simple method for the determination of coumarin (COU), 4-hydroxycoumarin (4HC) and dicoumarol (DC) in tea samples was needed, we developed an alternative option to chromatography, i.e., fluorescence spectroscopy with multivariate calibration. The synchronous fluorescence spectra were recorded at constant wavelength differences 70, 80 and 90 nm from 200 to 400 nm. The different experimental parameters affecting the synchronous fluorescence intensities of the analytes were carefully studied and optimized. Partial least squares (PLS) method and multi linear regression (MLR) were compared on determining the concentrations. The best results were obtained by the PLS method on synchronous fluorescence spectra at Δλ = 90 nm. The results from the analysis of herbal tea Melilotus officinalis by synchronous fluorescence spectroscopy with PLS model are equivalent with the results from HPLC. Fisher F- test and Student’s t- test confirmed this finding.

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Acknowledgments

This publication was supported by the Competence Center for SMART Technologies for Electronics and Informatics Systems and Services, ITMS 26240220072, funded by the Research&Development Operational Programme from the ERDF and the Slovak Research and Development Agency under the contract No. APVV-0797-11.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37, Bratislava, Slovakia

    Roman Poláček, Pavel Májek, Katarína Hroboňová & Jana Sádecká

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  1. Roman Poláček
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  2. Pavel Májek
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  3. Katarína Hroboňová
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  4. Jana Sádecká
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Correspondence to Roman Poláček.

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Poláček, R., Májek, P., Hroboňová, K. et al. Fluorescence Spectroscopy as a Tool for Determination of Coumarins by Multivariate Calibration. J Fluoresc 25, 297–303 (2015). https://doi.org/10.1007/s10895-015-1508-2

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  • DOI: https://doi.org/10.1007/s10895-015-1508-2

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