Journal of Fluorescence

, Volume 24, Issue 4, pp 1275–1284 | Cite as

“Is Macromolecular Crowding Overlooked?”- Effects of Volume Exclusion on DNA-Amino Acids Complexes and Their Reconstitutes

  • Priyanka D. Pal
  • Prabhakar M. Dongre
  • Arunkumar V. ChitreEmail author


Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers. The addition of high concentrations of natural and synthetic macromolecules to such buffers enables crowding to be mimicked in vitro, and should be encouraged as a routine variable to study. In this study, we propose to understand the changes in DNA character and its modulation in presence of macromolecules such as PEG with reference to binding parameters to amino acids using fluorescence enhancement.


Macromolecular crowding Fluorescence spectroscopy Binding constants DNA-amino acids 



Deoxyribose Nucleic Acid


Poly Ethylene Glycol


Ethidium Bromide


  1. 1.
    Ellis RJ (2001) Macromolecular crowding: obvious but underappreciated. Trends Biochem Sci 26:597–604PubMedCrossRefGoogle Scholar
  2. 2.
    Zimmerman SB, Minton AP (1993) Macromolecular crowding: biophysical, biochemical, and physiological consequences. Annu Rev Biophys Biomol Struct 22:27–65PubMedCrossRefGoogle Scholar
  3. 3.
    Marco J. Morelli, Rosalind J. Allen, and Pieter Rein ten Wolde; Effects of Macromolecular Crowding on Genetic Networks Biophys J. 2011 December 21; 101 (12): 2882–2891.Google Scholar
  4. 4.
    Zimmerman SB (1993) Macromolecular crowding effects on macromolecular interactions: some implications for genome structure and function. Biochim Biophys Acta 1216:175–185PubMedCrossRefGoogle Scholar
  5. 5.
    Zimmerman SB, Murphy LD (1996) Macromolecular crowding and the mandatory condensation of DNA in bacteria. FEBS Lett 390:245–248PubMedCrossRefGoogle Scholar
  6. 6.
    Peacocke AR, Skerrett JNH (1956) The interaction of aminoacridines with nucleic acids. Trans, Faraday Soc 52:261–79CrossRefGoogle Scholar
  7. 7.
    Olins DE, Olws AL, And PH, Hippel V (1967) Model Nucleoprotein Complexes: Studies on the Interaction of Cationic Homopolypeptides with DNA; J. Mol Biol 24:157–176CrossRefGoogle Scholar
  8. 8.
    Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. New York Acad. Sci. 51: 660–672Google Scholar
  9. 9.
    Irvin JL, Irvin EM (1954) The interaction of quinacrine withadenine nucleotides. J Biol Chem 210:45–56PubMedGoogle Scholar
  10. 10.
    Nakano S-i, Miyoshi D, Sugimoto N (2014) Effects of Molecular Crowding on the Structures, Interactions, and Functions of Nucleic Acids. Chem Rev 114:2733–2758PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Priyanka D. Pal
    • 1
  • Prabhakar M. Dongre
    • 1
  • Arunkumar V. Chitre
    • 1
    Email author
  1. 1.Department of BiophysicsUniversity Of MumbaiMumbaiIndia

Personalised recommendations