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Journal of Fluorescence

, Volume 24, Issue 4, pp 1275–1284 | Cite as

“Is Macromolecular Crowding Overlooked?”- Effects of Volume Exclusion on DNA-Amino Acids Complexes and Their Reconstitutes

  • Priyanka D. Pal
  • Prabhakar M. Dongre
  • Arunkumar V. ChitreEmail author
ORIGINAL PAPER

Abstract

Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers. The addition of high concentrations of natural and synthetic macromolecules to such buffers enables crowding to be mimicked in vitro, and should be encouraged as a routine variable to study. In this study, we propose to understand the changes in DNA character and its modulation in presence of macromolecules such as PEG with reference to binding parameters to amino acids using fluorescence enhancement.

Keywords

Macromolecular crowding Fluorescence spectroscopy Binding constants DNA-amino acids 

Abbreviations

DNA

Deoxyribose Nucleic Acid

PEG

Poly Ethylene Glycol

EB

Ethidium Bromide

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Priyanka D. Pal
    • 1
  • Prabhakar M. Dongre
    • 1
  • Arunkumar V. Chitre
    • 1
    Email author
  1. 1.Department of BiophysicsUniversity Of MumbaiMumbaiIndia

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