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A Fluorescent Chemodosimeter for Hg2+Based on a Spirolactam Ring-Opening Strategy and its Application Towards Mercury Determination in Aqueous and Cellular Media

Journal of Fluorescence volume 24, pages 67–74 (2014)Cite this article

Abstract

A novel fluorescent chemosensor rhodamine B phenyl hydrazide (RBPH) for Hg2+ was designed and synthesized. This probe is highly sensitive, selective, and irreversible for Hg2+ and exhibits fluorescent response at 580 nm. RBPH also displayed detectable color change from colorless to pink upon treatment with Hg2+. This property has been utilized as naked eye detection for Hg2+ in various industrial samples. Fluorescence microscopic experiments demonstrated that this chemosensor is cell permeable and can be used for fluorescence imaging of Hg2+ in cellular media. This probe can detect Hg2+ with good linear relationships from 1 to 100 nM with r = 0.99983 and the limit of detection were found to be 0.019 nM with ± 0.91 % RSD at 10 nM concentrations.

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Acknowledgments

The authors acknowledge the Jain University, Bangalore, India for financial assistance and Bangalore University for providing the CHN analyzer facility and Indian Institute of Science, Bangalore, for providing NMR and ESI-MS facility.

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Authors and Affiliations

  1. Center for Nano and Material Sciences, Global Campus, Jain University, Jakkasandra (P), Kankapura (T), Bangalore, 560001, Karnataka, India

    Kempahanumakkagaari Suresh Kumar, Thippeswamy Ramakrishnappa & R. Geetha Balakrishna

  2. Department of Studies in Chemistry, Central College Campus, Dr. Ambedkar Veedi, Bangalore University, Bangalore, 560 001, India

    Mallingappagari Pandurangappa

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  1. Kempahanumakkagaari Suresh Kumar
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  2. Thippeswamy Ramakrishnappa
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  3. R. Geetha Balakrishna
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  4. Mallingappagari Pandurangappa
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Correspondence to Thippeswamy Ramakrishnappa.

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Kumar, K.S., Ramakrishnappa, T., Balakrishna, R.G. et al. A Fluorescent Chemodosimeter for Hg2+Based on a Spirolactam Ring-Opening Strategy and its Application Towards Mercury Determination in Aqueous and Cellular Media. J Fluoresc 24, 67–74 (2014). https://doi.org/10.1007/s10895-013-1271-1

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