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β-cyclodextrin Sensitized Spectrofluorimetry for the Determination of Abiraterone Acetate and Abiraterone

Abstract

A novel spectrofluorimetric method to determine abiraterone acetate and its active metabolite, abiraterone was developed, based on the fact that fluorescence intensity of abiraterone acetate and abiraterone could be enhanced in β-cyclodextrin (β-CD) due to the formation of the inclusion complex. The inclusion interaction of β-CD and abiraterone acetate and the β-cyclodextrin sensitized spectrofluorimetry was examined. The various factors influencing fluorescence were discussed in details. The results showed that under the optimized conditions, the linear range of calibration curve for the determination of biraterone acetate and abiraterone was 0.20 ~ 6.0 μg/mL, and the detection limit (LOD) was 6.8 (r = 0.997) or 6.6 ng/mL (r = 0.996), respectively. No interference was observed from common co-existing substances or pharmaceutical excipient. The method was successfully applied to the analysis of abiraterone acetate in pharmaceutical formulation and abiraterone in human serum/urine.

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Acknowledgments

The authors acknowledge the financial support from the National Natural Science Foundation of China (20875082, 21155001) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Foundation of the Excellence Science and Technology Invention Team in Yangzhou University

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Correspondence to Xiashi Zhu.

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Gong, A., Zhu, X. β-cyclodextrin Sensitized Spectrofluorimetry for the Determination of Abiraterone Acetate and Abiraterone. J Fluoresc 23, 1279–1286 (2013). https://doi.org/10.1007/s10895-013-1261-3

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  • DOI: https://doi.org/10.1007/s10895-013-1261-3

Keywords

  • Abiraterone acetate
  • Abiraterone
  • β-cyclodextrin
  • Sensitized spectrofluorimetry