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A Graphene Oxide–Based Sensing Platform for The Label-free Assay of DNA Sequence and Exonuclease Activity via Long Range Resonance Energy Transfer

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Abstract

Graphene oxide (GO) was introduced as an efficient quencher for label-free and sensitive detection of DNA. Probe DNA (pDNA) was mixed with ethidium bromide (EB) and graphene oxide (GO). The interaction between EB and GO led to the fluorescent quenching. Upon the recognition of the target, EB was intercalated into duplex DNA and kept away from GO, which significantly hindered the long range resonance energy transfer (LrRET) from EB to GO and, thus, increased the fluorescence of EB. The changes in fluorescent intensity produced a novel method for sensitivity, and specificity detection of the target. Based on the structure-switching of aptamers, this strategy could be conveniently extended for detection of other biomolecules, which had been demonstrated by the detection of exonuclease activity.

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Abbreviations

GO:

Graphene oxide

pDNA:

Probe DNA

EB:

Ethidium bromide

LrRET:

Long range resonance energy transfer

Exo III:

Exonuclease

tDNA:

Target DNA

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Acknowledgments

This work has been supported by National Natural Science Foundation of China (No. 21165004, 21163002), the Guangxi Natural Science Foundation of China (2010GXNSFF013001, 2012GXNSFBA053022), Innovation Plan in Graduate Education of Guangxi Province (2010106020703 M70) and the project of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University), Ministry of Education of China(CMEMR2011-14).

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Authors and Affiliations

  1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, 541004, China

    Yixuan Jiang, Jianniao Tian, Sheng Chen, Yanchun Zhao, Yuan Wang & Shulin Zhao

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  1. Yixuan Jiang
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  2. Jianniao Tian
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  3. Sheng Chen
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  4. Yanchun Zhao
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  5. Yuan Wang
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  6. Shulin Zhao
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Correspondence to Jianniao Tian.

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Jiang, Y., Tian, J., Chen, S. et al. A Graphene Oxide–Based Sensing Platform for The Label-free Assay of DNA Sequence and Exonuclease Activity via Long Range Resonance Energy Transfer. J Fluoresc 23, 697–703 (2013). https://doi.org/10.1007/s10895-013-1189-7

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  • DOI: https://doi.org/10.1007/s10895-013-1189-7

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