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Validated HPLC-Fl Method for the Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine Biomarkers in Human Blood

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Abstract

The natural methyl donor group, S-adenosylmethionine and its product, S-adenosylhomocysteine play an important role in many biochemical reactions involving transmethylation reactions. These compounds can be used as biomarkers in incipient diagnosis of various pathological disorders therefore the validation of a suitable method to routinely analysis of these compounds is very important. In this paper, a high performance liquid chromatrography method for S-adenosylmethionine and S-adenosylhomocysteine measurement as fluorescent 1,N 6-ethanoderivatives from biological samples was validated in terms of selectivity, linearity range of the response (R > 0.9993), detection limit (9 × 10−9 and 4.4 × 10−9 molL−1), the limit of quantitation (9.7 × 10−9 and 5.7 × 10−9 mol L−1), precision, trueness and robustness. The method for quantification simultaneous of these compounds is rapid, sensitive and precise and appropriate for clinical analysis.

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Acknowledgments

The authors are grateful to Romanian Ministry of Education and Research (research grant PN 09–360101, BIODIV).

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Authors and Affiliations

  1. Centre of Bioanalysis, National Institute for Biological Sciences, Bucharest, 296 Spl. Independentei, Bucharest, Romania

    Camelia Albu & Simona Carmen Litescu

  2. Faculty of Applied Chemistry and Material Science, Politechnica University of Bucharest, Bucharest, 1-7 Polizu Str, Bucharest, Romania

    Gabriel Lucian Radu

  3. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, 12311, Egypt

    Hassan Y. Aboul-Enein

Authors
  1. Camelia Albu
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  2. Simona Carmen Litescu
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  3. Gabriel Lucian Radu
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  4. Hassan Y. Aboul-Enein
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Corresponding author

Correspondence to Hassan Y. Aboul-Enein.

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Albu, C., Litescu, S.C., Radu, G.L. et al. Validated HPLC-Fl Method for the Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine Biomarkers in Human Blood. J Fluoresc 23, 381–386 (2013). https://doi.org/10.1007/s10895-013-1173-2

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  • DOI: https://doi.org/10.1007/s10895-013-1173-2

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