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Optimization of Fluoroimmunoassay against C-Reactive Protein Exploiting Immobilized-antigen Glass Slide

Journal of Fluorescence volume 23, pages 243–249 (2013)Cite this article

Abstract

An optimization experiment for an indirect-competitive (IC) fluoroimmunoassay (FIA) against C-reactive protein (CRP) was conducted exploiting an immobilized-antigen glass slide and an anti-CRP antibody tagged with fluorescent silica nanoparticles (FSNPs). The optimized conditions for the IC FIA were as follows: time and concentration of treatment with glutaraldehyde, 30 min and 1.5 %, respectively; time of reaction with coating antigen and concentration of coating antigen for immobilization, 1 h and 0.1 mg/mL, respectively; concentration of FSNP-anti-CRP antibody conjugate coupled by the biotin-avidin interaction, the bioconjugate, for immune reaction, 0.250 mg/mL; concentration of bovine serum albumin (BSA) for blocking and time of blocking with BSA, 3 % and 30 min, respectively. By using the glass slide, a highly sensitive detection against CRP was possible with the limit of detection below 0.1 ng/mL.

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Acknowledgements

This study was carried out as one part of the research project of Development of Food Nanotechnology, Korea Food Research Institute, Republic of Korea.

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  1. Functional Materials Research Group, Korea Food Research Institute, Songnam, 463-746, Republic of Korea

    Namsoo Kim & Yong-Jin Cho

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  1. Namsoo Kim
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  2. Yong-Jin Cho
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Correspondence to Namsoo Kim.

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Kim, N., Cho, YJ. Optimization of Fluoroimmunoassay against C-Reactive Protein Exploiting Immobilized-antigen Glass Slide. J Fluoresc 23, 243–249 (2013). https://doi.org/10.1007/s10895-012-1140-3

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  • DOI: https://doi.org/10.1007/s10895-012-1140-3

Keywords

  • Optimization
  • Indirect-competitive fluoroimmunoassay
  • C-Reactive protein
  • Antibody tagged with fluorescent silica nanoparticles
  • Immobilized-antigen glass slide