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Europium Coordination Complexes as Potential Anticancer Drugs: Their Partitioning and Permeation Into Lipid Bilayers as Revealed by Pyrene Fluorescence Quenching

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Abstract

The present study was undertaken to evaluate the membrane-associating properties of a series of novel antitumor agents, Eu(III) coordination complexes (EC), using the pyrene fluorescence quenching as an analytical instrument. Analysis of EC-induced decrease in pyrene fluorescence intensity in terms of partition and solubility-diffusion models allowed us to evaluate the partition and permeation coefficients of the examined compounds into the lipid vesicles prepared from zwitterionic lipid phosphatidylcholine (PC) and its mixtures with cholesterol (Chol) and anionic lipid cardiolipin (CL). The drug-lipid interactions were found to have the complex nature determined by both EC structure and lipid bilayer composition. High values of the obtained partition and permeation coefficients create the background for the development of EC liposomal formulations.

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Acknowledgments

This work was supported by the grant from Fundamental Research State Fund (project number F.41.4/014) and young scientist award by the President of Ukraine to VT (project number GP/F32/109).

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Authors and Affiliations

  1. Department of Nuclear and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077, Ukraine

    Valeriya Trusova, Andrey Yudintsev, Ludmila Limanskaya & Galyna Gorbenko

  2. Department of Applied Organic Chemistry, Faculty of Chemistry and Pharmacy, University of Sofia, Sofia, 1164, Bulgaria

    Todor Deligeorgiev

  3. 19-32 Geroyev Truda Str., Kharkov, 61144, Ukraine

    Valeriya Trusova

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  1. Valeriya Trusova
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Correspondence to Valeriya Trusova.

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Trusova, V., Yudintsev, A., Limanskaya, L. et al. Europium Coordination Complexes as Potential Anticancer Drugs: Their Partitioning and Permeation Into Lipid Bilayers as Revealed by Pyrene Fluorescence Quenching. J Fluoresc 23, 193–202 (2013). https://doi.org/10.1007/s10895-012-1134-1

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