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New Luminescent Bioprobes Eu(lll)-phloroglucinol Derivatives and Their Spectrofluorimetric, Electrochemical Interactions with Nucleotides and DNA

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Abstract

Two new ligands derived from phloroglucinol 2-{[(4-methoxy benzoyl ) oxy ] } methyl benzoic acid[L1] and 2-{[(4-methyl benzoyl )oxy] methyl} benzoic acid[L2] were synthesized. The solid complex Eu(III)-L2 has been synthesised and characterized by elemental analysis ,UV and IR spectra. The reaction of Eu(III) with the two synthesized ligands has been investigated in I = 0.1 mol dm-3 p-toluene sulfonate by cyclic voltammetry and square wave voltammetry. The reaction of Eu (III)–L1 and Eu (III)–L2 binary complexes with nucleotide 5′-AMP , 5′-ADP ,5′-ATP , 5′- GMP , 5′-IMP , and 5′-CMP has been investigated using UV, fluorescence and electrochemical methods. The experimental conditions were selected such that self-association of the nucleotides and their complexes was negligibly small, that is, the monomeric complexes were studied. The interaction of the Eu(III)–L1 or L 2 solid complexes with calf-thymus DNA has been investigated by fluorescence and electrochemical methods including cyclic voltammetery(CV) ,differential pulse polarography (DPP) and square wave voltammetry (SWV) on a glassy carbon electrode. The fluorescence intensity of Eu(III)-L2 complex was enhanced with the addition of DNA. Under optimal conditions in phosphate buffer pH 7.0 at 25 °C the linear range is 3–20 μM for calf thymus DNA (CT–DNA) and the corresponding determination limit is 1.8 μM.

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Authors and Affiliations

  1. Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt

    Hassan Ahmed Azab, Zeinab M. Anwar, Enas T. Abdel-Salam & Mahmoud EL-Sayed-Sebak

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  1. Hassan Ahmed Azab
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  2. Zeinab M. Anwar
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  3. Enas T. Abdel-Salam
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  4. Mahmoud EL-Sayed-Sebak
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Correspondence to Hassan Ahmed Azab.

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Azab, H.A., Anwar, Z.M., Abdel-Salam, E.T. et al. New Luminescent Bioprobes Eu(lll)-phloroglucinol Derivatives and Their Spectrofluorimetric, Electrochemical Interactions with Nucleotides and DNA. J Fluoresc 22, 223–238 (2012). https://doi.org/10.1007/s10895-011-0949-5

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  • DOI: https://doi.org/10.1007/s10895-011-0949-5

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