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Bioconjugated Fluorescent Polymeric Nanoparticles for Imaging and Targeted Therapy of HER2-Overexpressing Cancer Cells

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Abstract

We report the development of Herceptin-conjugated fluorescent polymeric nanoparticles (PNp) probes. Synthesis of fluorescent conjugated polymer as the core, preparation of the core/shell PNp, the ability of immobilizing Herceptin on PNp, targeting and imaging of bioconjugated PNp toward HER2-overexpressing cancer cells, and therapeutic effect on cell cycle, together with the expression of apoptosis related proteins, were investigated. We have achieved active tumor targeting by rapid PNp-antibody binding to tumor-specific antigens. Besides, Herceptin-conjugated PNp can suppress the growth of HER2-overexpressing cancer cells.

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Acknowledgements

The authors would like to thank the financial support from the National Science Council, Feng Chia University and China Medical University.

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Authors and Affiliations

  1. Department of Chemical Engineering, Feng Chia University, 100, Wenhwa Road, Seatwen, Taichung, 40724, Taiwan

    Chi-Jung Chang

  2. Department of Biological Science and Technology, College of Life Sciences, China Medical University, 91, Hsueh-Shih Road, Taichung, 40402, Taiwan

    Tzong-Der Way

  3. Department of Medical Laboratory Science and Biotechnology, China Medical University, 91, Hsueh-Shih Road, Taichung, 40402, Taiwan

    Cheng-Wen Lin

Authors
  1. Tzong-Der Way
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  2. Chi-Jung Chang
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  3. Cheng-Wen Lin
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Correspondence to Chi-Jung Chang.

Additional information

Chi-Jung Chang and Tzong-Der Way contributed equally to this work.

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Way, TD., Chang, CJ. & Lin, CW. Bioconjugated Fluorescent Polymeric Nanoparticles for Imaging and Targeted Therapy of HER2-Overexpressing Cancer Cells. J Fluoresc 21, 1669–1676 (2011). https://doi.org/10.1007/s10895-011-0856-9

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  • DOI: https://doi.org/10.1007/s10895-011-0856-9

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