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Fluorescence Imaging of Stem Cells, Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots

Abstract

Trioctylphosphine oxide capped cadmium selenide quantum dots, synthesized in organic media were rendered water soluble by silica overcoating. Silanisation was done by a simple reverse microemulsion method using aminopropyl silane as the silica precursor. Further, the strong photoluminescence of the silica-coated CdSe quantum dots has been utilized to visualize rabbit adipose tissue-derived mesenchymal stem cells (RADMSCs) and Daltons lymphoma ascites (DLA) cancerous cells in vitro. Subsequently the in vivo fluorescence behaviours of QDs in the tissues were also demonstrated by intravenous administration of the QDs in Swiss albino mice. The fluorescence microscopic images in the stem cells, cancer cells and semi-thin sections of mice organs proved the strong luminescence property of silica-coated quantum dots under biological systems. These results establish silica-coated CdSe QDs as extremely useful tools for molecular imaging and cell tracking to study the cell division and metastasis of cancer and other diseases.

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Acknowledgements

We gratefully acknowledge the Department of Biotechnology, Ministry of Science and Technology, Govt. of India, New Delhi, for the financial assistance as research grant (Order No.BT/PR9904/ NNT/28/63/2007) given to Dr. Annie Abraham, Principal Investigator, DBT project; Dr. K. George Thomas, Scientist, National Institute of Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, India for supplying nanomaterials; UGC, Govt. of India for the research fellowship to Vinayakan R.

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Correspondence to Annie Abraham.

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Vibin, M., Vinayakan, R., John, A. et al. Fluorescence Imaging of Stem Cells, Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots. J Fluoresc 21, 1365–1370 (2011). https://doi.org/10.1007/s10895-010-0818-7

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Keywords

  • DLA cells
  • Luminescence
  • Quantum dots
  • RADMSCs
  • Semi-thin section