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Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe

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Abstract

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

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Acknowledgments

This work was supported by the National Institutes of Health (1 R15 EB008858-01), the National Science Foundation (CHE-0832622 and CHE-0840431), the US Civilian Research and Development Foundation (UKB2-2923-KV-07), and the Ministry of Education and Science of Ukraine (grant M/49-2008).

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Authors and Affiliations

  1. Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL, 32816, USA

    Carolina D. Andrade, Ciceron O. Yanez, Maher A. Qaddoura, Xuhua Wang, Curtesa L. Arnett, Sabrina A. Coombs, Jin Yu, Rania Bassiouni & Kevin D. Belfield

  2. CREOL, The College of Optics and Photonics, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL, 32816, USA

    Kevin D. Belfield

  3. Department of Photoactivity, Institute of Physics, National Academy of Sciences of Ukraine Prospect Nauki 46, Kiev, Ukraine

    Mykhailo V. Bondar

Authors
  1. Carolina D. Andrade
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  2. Ciceron O. Yanez
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  3. Maher A. Qaddoura
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  4. Xuhua Wang
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  5. Curtesa L. Arnett
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  6. Sabrina A. Coombs
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  7. Jin Yu
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  8. Rania Bassiouni
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  9. Mykhailo V. Bondar
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  10. Kevin D. Belfield
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Corresponding author

Correspondence to Kevin D. Belfield.

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Andrade, C.D., Yanez, C.O., Qaddoura, M.A. et al. Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe. J Fluoresc 21, 1223–1230 (2011). https://doi.org/10.1007/s10895-010-0801-3

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  • DOI: https://doi.org/10.1007/s10895-010-0801-3

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