Skip to main content
SpringerLink
Log in

Spectrofluorimetric Determination of Coumarin in Commercial Tablets

  • Original Paper
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

A simple, rapid and effective analytical method based on fluorescence spectroscopy for the determination of coumarin in pharmaceutical formulations without pre-treatment or pre-concentration step was development. Coumarin had maximum excitation and emission at 310 nm and 390 nm, respectively. Optimum conditions for the detection of coumarin were investigated. Under optimized conditions, we observed a linear behavior for the sign of coumarin in the concentration range of 2.5 × 10−6 to 1.0 × 10−4 mol L−1, with linearity of 0.998 and sensitivity of 2.9 × 1010 u.a/mol L−1. The proposed method was validated in terms of accuracy, precision and specificity of coumarin using the standard addition and external calibration. It was noted that the results support (P < 0.05), indicating that the matrices were not an interference in the determination of coumarin by fluorescence spectroscopy. The results were favorable compared with those obtained by reference chromatographic methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Raters M, Matissek R (2008) Analysis of coumarin in various foods using liquid chromatography with tandem mass spectrometric detection. Eur Food Res Technol 227:637–642

    Article  CAS  Google Scholar 

  2. http://libdigi.unicamp.br/document/?code=vtls000381835, accessed in August 2009.

  3. Oldenburg J, Rost S, Seidel H, Watzka M, Mueller-Reible CR (2008) Pharmakogenetik der oralen Antikoagulation mit Cumarinen. Medizin Gen 20(2):230–235

    Article  CAS  Google Scholar 

  4. Hoult JRS, Payá M (1996) Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol 27(4):713–722

    PubMed  CAS  Google Scholar 

  5. Hamerschlak N, Rosenfeld LGM (1996) Utilização da heparina e dos anticoagulantes orais na prevenção e tratamento da trombose venosa profunda e da embolia pulmona. Arq Bras Cardiol 67(3):2909–213

    Google Scholar 

  6. Beckley-Kartey SAJ, Hotchkiss SAM, Capel M (1997) Comparative in vitro skin absorption and metabolism of coumarin (1, 2-benzopyrone) in human rat. Toxicol App Pharm 145(1):34–42

    Article  CAS  Google Scholar 

  7. Nie J, Wu H, Zhu S, Han Q, Fu H (2008) Simultaneous determination of 6-methylcoumarin and 7-methoxycoumarin in cosmetics using three-dimensional excitation–emission matrix fluorescence coupled with second-order calibration methods. Talanta 75:1260–1268

    Article  PubMed  CAS  Google Scholar 

  8. Rychlik M (2008) Quantification of free coumarin and its liberation from glucosylated precursors by stable isotope dilution assays based on liquid chromatography-tandem mass spectrometric detection. J Agricul Food Chem 56:796–801

    Article  CAS  Google Scholar 

  9. Jager LS, Perfetti GA, Diachenko GW (2007) Determination of coumarin, vanillin, and ethyl vanillin in vanilla extract products: liquid chromatography mass spectrometry method development and validation studies. J Chromatogr A 1145:83–88

    Article  PubMed  Google Scholar 

  10. Janegitz BC, Suarez WT, Fatibello-Filho O, Marcolino-Junior LH (2008) Conductometric determination of n-acetylcysteine in pharmaceutical formulations using copper(ii) sulphate as titrant. Anal Lett 41:3264–3269

    Article  CAS  Google Scholar 

  11. Pedrosa VA, Malagutti AR, Mazo LH, Avaca LA (2006) The use of boron-doped diamond electrodes for the amperometric determination of flavonoids in a flow injection system. Anal Lett 39:2737–2748

    Article  CAS  Google Scholar 

  12. Finn GJ, Creaven BS, Egan DA (2004) A study of the role of cell cycle events mediating the action of coumarin derivatives in human malignant melanoma cells. Cancer Lett 214(1):43–54

    Article  PubMed  CAS  Google Scholar 

  13. Fery-Forgues S, El-Ayoubi R, Lamère J (2008) Fluorescent microcrystals obtained from coumarin 6 using the reprecipitation method. J Fluoresc 18:619–624

    Article  PubMed  CAS  Google Scholar 

  14. Ikeda R, Wada M, Nishigaki T, Nakashima K (2009) Quantification of coumarin derivatives in Noni (Morinda citrifolia) and their contribution of quenching effect on reactive oxygen species. Food Chem 113:1169–1172

    Article  CAS  Google Scholar 

  15. Yip RW, Wen YX, Szabo AG (1993) Decay associated fluorescence spectra of coumarin 1 and coumarin 102: evidence for a two-state solvation kinetics in organic solvents. J Phys Chem 97:10458–10462

    Article  CAS  Google Scholar 

  16. Mannekutla JR, Mulimani BG, Inamdar SR (2008) Solvent effect on absorption and fluorescence spectra of coumarin laser dyes: evaluation of ground and excited state dipole moments. Spectrochim Acta, Part A 69:419–426

    Article  CAS  Google Scholar 

  17. Satpati A, Senthilkumar S, Kumbhaka M, Nath S, Maity DK, Pal H (2005) Investigations of the solvent polarity effect on the photophysical properties of coumarin-7 dye. Photochem Photobiol 81:270–278

    Article  PubMed  CAS  Google Scholar 

  18. Lakowicz JR (2006) Principles of fluorescence spectroscopy. Springer, New York

    Book  Google Scholar 

  19. Shabir GA (2003) Validation of high-performance chromarography methods for pharmaceutical analysis. Understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International conference on harmonization. J Chromatogr A 987(1–2):57–63

    Article  PubMed  CAS  Google Scholar 

  20. Miller JC, Miller JN (1993) (1993) Statistics for Analytical Chemistry. Ellis Horwood Limited, New York

    Google Scholar 

  21. Celeghini RMS, Vilegas JHY, Lanças FM (2001) Extraction and quantitative hplc analysis of coumarin in hydroalcoholic extracts of mikania glomerata spreng. (“guaco”) leaves. J Braz Chem Soc 12(6):706–709

    Article  CAS  Google Scholar 

  22. Ochacka RJ, Rajzer D, Kowalski P, Lamparczyk H (1995) Determination of coumarins from Chrysanthemum segetum L. by capillary electrophoresis. J Chromatogr A 709:197–202

    Article  Google Scholar 

  23. Cohen AJ (1979) Critical review of the toxicology of coumarin with special reference to interspecies diferences in metabolism and hepatotoxic response and their signicance to man. Food Chem Toxicol 17:277–289

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank CNPq (479655/2008-1), FAPESP (08/50588-6 and 06/56701-3), and L´Oreal/Academia Brasileira de Ciências/Unesco (Grant for Woman in Science 2007) for the financial support.

Author information

Authors and Affiliations

  1. Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, São José dos Campos, SP, Brazil

    Mirian Marcolan & Priscila Alfonso Martins

  2. Department of Chemistry and Biochemistry, Institute of Bioscience, UNESP, Botucatu, SP, Brazil

    Valber A. Pedrosa

  3. Departamento de Ciência e Tecnologia, Universidade Federal Fluminense, Polo Universitário de Rio das Ostras, Rio das Ostras, RJ, Brazil

    Maira R. Rodrigues

  4. Universidade Camilo Castelo Branco, Núcleo Parque Tecnológico, Rodovia Presidente Dutra, Km 138, 12247-004, São José dos Campos, SP, Brazil

    Hueder P. M. de Oliveira

  5. Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo - Campus Diadema, Rua Prof. Artur Riedel, 275 - Bairro Eldorado, Diadema, SP, Brazil, CEP: 09972-270

    Lucia Codognoto

Authors
  1. Mirian Marcolan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Priscila Alfonso Martins
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Valber A. Pedrosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maira R. Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hueder P. M. de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lucia Codognoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lucia Codognoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marcolan, M., Martins, P.A., Pedrosa, V.A. et al. Spectrofluorimetric Determination of Coumarin in Commercial Tablets. J Fluoresc 21, 733–738 (2011). https://doi.org/10.1007/s10895-010-0763-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-010-0763-5

Keywords

Search

Navigation