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Fluorescence Enhancement of the Silver Nanoparticales – Curcumin - Cetyltrimethylammonium Bromide-nucleic Acids System and its Analytical Application

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Abstract

It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU) - cetyltrimethylammonium bromide (CTAB) – nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 2.0 × 10−8–1.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 2.0 × 10−8–1.0 × 10−6 g mL−1 for calf thymus DNA (ctDNA), 1.0 × 10−8–1.0 × 10−6 g mL−1 for yeast RNA (yRNA), and their detection limits (S/N = 3) are 8.0 ng mL−1, 10.5 ng mL−1 and 5.8 ng mL−1, respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The interaction mechanism is also studied.

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Acknowledgment

This work is supported by Natural Science Foundations of China (20575035) and Shandong Province(Z2008B04).

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Authors and Affiliations

  1. Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China

    Haiping Zhou, Xia Wu, Wei Xu & Jinghe Yang

  2. School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Qiuxia Yang

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  1. Haiping Zhou
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  2. Xia Wu
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  3. Wei Xu
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  4. Jinghe Yang
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Correspondence to Xia Wu or Qiuxia Yang.

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Zhou, H., Wu, X., Xu, W. et al. Fluorescence Enhancement of the Silver Nanoparticales – Curcumin - Cetyltrimethylammonium Bromide-nucleic Acids System and its Analytical Application. J Fluoresc 20, 843–850 (2010). https://doi.org/10.1007/s10895-010-0627-z

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  • DOI: https://doi.org/10.1007/s10895-010-0627-z

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