Abstract
Understanding the sequence dependent molecular recognition of DNA is crucial for the rational design of many drugs. Femtosecond resolved studies on the hydration dynamics of the dodecamer duplexes having sequences (CGCGAATTCGCG)2 and (CGCAAATTTGCG)2, and their complexes with the nucleic protein histone 1 (H1) reveal significant correlation of the molecular recognition of the DNA and DNA-protein complexes with the dynamics of hydration. The different molecular recognition of DNA and DNA-protein complexes is also borne out by circular dichroism (CD) and fluorescence detected CD measurements.
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Acknowledgement
DB, AM thanks CSIR, India for fellowship. We thank DST for financial grant (SR/SO/BB-15/2007).
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Banerjee, D., Makhal, A. & Pal, S.K. Sequence Dependent Femtosecond-Resolved Hydration Dynamics in the Minor Groove of DNA and Histone—DNA Complexes. J Fluoresc 19, 1111–1118 (2009). https://doi.org/10.1007/s10895-009-0512-9
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DOI: https://doi.org/10.1007/s10895-009-0512-9