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Fluorescent Biological Label for Recognizing Human Ovarian Tumor Cells Based on Fluorescent Nanoparticles

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Abstract

In this paper, we report a method for recognizing human ovarian tumor(HOT) cells using fluorescent biological label based on core-shell nanoparticles. The luminescent nanoparticles were synthesized with a water-in-oil(W/O)micromulsion technique. The fluorescent silica core-shell nanoparticles modified with anti-HER2 antibody using bifunctional cross-linker glutaraldedhyde targeted the corresponding tumor antigen in the cell surface of the SKOV3 ovarian cancer cells. The specific immunoreactivity of antibody-nanoparticles with cells was characterized by laser scanning microscopy (LSM) and scanning electron microscope (SEM). The results showed that the method offered potential advantages of sensitivity and simplicity due to high binding efficiency between nanoparticles and cells and provided an alternative method for the detection of HOT.

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Acknowledgments

This work was supported by the National High-tech R&D program (863 program, 2007AA06Z402), Project of the Foundation of Shanghai Municipal Government (08520510400), Shanghai Leading Academic Discipline Project (S30406) and Leading Academic Discipline Project of Shanghai Normal University(DZL706).

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Authors and Affiliations

  1. College of Life and Environment, Shanghai Normal University, Shanghai, 200234, China

    Shasheng Huang, Ruina Li, Yongxia Qu, Jian Shen & Jia Liu

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  1. Shasheng Huang
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  2. Ruina Li
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  3. Yongxia Qu
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  4. Jian Shen
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  5. Jia Liu
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Correspondence to Shasheng Huang.

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Huang, S., Li, R., Qu, Y. et al. Fluorescent Biological Label for Recognizing Human Ovarian Tumor Cells Based on Fluorescent Nanoparticles. J Fluoresc 19, 1095–1101 (2009). https://doi.org/10.1007/s10895-009-0509-4

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  • DOI: https://doi.org/10.1007/s10895-009-0509-4

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