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Spectrofluorimetric Determination of Famotidine in Pharmaceutical Preparations and Biological Fluids. Application to Stability Studies

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Abstract

A simple, economic, selective, and stability indicating spectrofluorimetric method was developed for the determination of famotidine (FMT); is based on its reaction with 9, 10-phenanthraquinone in alkaline medium to give a highly fluorescent derivative measured at 560 nm after excitation at 283 nm. The fluorescence intensity - concentration plot was rectilinear over the concentration range of 50–600 ng/ml with minimum quantification limit (LOQ) of 13.0 ng/ml and minimum detection limit (LOD) of 4.3 ng/ml. The factors affecting the development of the fluorescence intensity of the reaction product were carefully studied and optimized. The method was applied for the determination of FMT in its dosage forms. The stability of the compound was studied, and the proposed method was found to be stability indicating one. The results obtained were in good agreement with those obtained by the official method. Furthermore, the method was applied for the determination of FMT in spiked and real human plasma. The mean % recovery (n = 4) was found to be 99.94 ± 0.24, and 105.13 ± 0.64 for spiked and real human plasma, respectively. The composition of the reaction product as well as its stability constant was also investigated. Moreover, the method was utilized to investigate the kinetics of both alkaline and oxidative induced degradation of the drug. The apparent first order rate constant and half life time of the degradation product was calculated. A proposal of the reaction pathway was postulated.

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References

  1. Reynolds JEF (1999) Martindale: the extra pharmacopoeia, 32rd edn. The Pharmaceutical Press, Massachusetts, pp 810–812

    Google Scholar 

  2. Apostu M, Bibire N, Dorneanu V (2005) UV spectrophotometric assay of famotidine in combination with picrolonic acid, picrolinate. Med Chir Soc Med Nat Iasi 109:422–425

    CAS  Google Scholar 

  3. Rahman N, Kashif M (2003) Application of ninhydrin to spectrophotometric determination of famotidine in drug formulations. Farmaco 58:1045–1050 doi:10.1016/S0014-827X(03)00184-8

    Article  PubMed  CAS  Google Scholar 

  4. Rahman N, Kashif M (2003) Kinetic spectrophotometric determination of famotidine in commercial dosage forms. Anal Sci 19:907–911 doi:10.2116/analsci.19.907

    Article  PubMed  CAS  Google Scholar 

  5. Ayad MM, Shalaby A, Abdellatef HE, Hosny MM (2003) New colorimetric methods for the determination of trazodone HCl, famotidine, and diltiazem HCl in their pharmaceutical dosage forms. Anal Bioanal Chem 376:710–714 doi:10.1007/s00216-003-1954-6

    Article  PubMed  CAS  Google Scholar 

  6. Barańska M, Gumienna-Kontecka E, Kozlowski H, Proniewicz LM (2002) A study on the nickel II-famotidine complexes. J Inorg Biochem 92:112–120 doi:10.1016/S0162-0134(02)00485-3

    Article  PubMed  Google Scholar 

  7. Al-Ghannam S, Belal F (2002) Spectrophotometric determination of three anti-ulcer drugs through charge-transfer complexation. J AOAC Int 85:1003–1008

    PubMed  CAS  Google Scholar 

  8. Chukwurah BK, Ajali U (2001) Quantitative determination of famotidine through charge-transfer complexation with chloranilic acid. Boll Chim Farm 140:354–360

    PubMed  CAS  Google Scholar 

  9. Walash MI, Sharaf-El-Din MK, Metwally ME, Shabana MR (2005) Kinetic spectrophotometric determination of famotidine in pharmaceutical preparations. J Chin Chem Soc 52:71–76

    CAS  Google Scholar 

  10. Walash MI, Sharaf-El-Din MK, Metwally ME, Shabana MR (2005) Polarographic determination of famotidine through complexation with Nickel (II) Chloride. J Chin Chem Soc 52:927–935

    CAS  Google Scholar 

  11. Helali N, Monser L (2008) Stability indicating method for famotidine in pharmaceuticals using porous graphitic carbon column. J Sep Sci 31:276–282 doi:10.1002/jssc.200700347

    Article  PubMed  CAS  Google Scholar 

  12. Ashiru DA, Patel R, Basit A (2007) A Simple and universal HPLC-UV method to determine cimetidine, ranitidine, famotidine and nizatidine in urine: application to the analysis of ranitidine and its metabolites in human volunteers. J Chromatogr B Analyt Technol Biomed Life Sci 860:235–240 doi:10.1016/j.jchromb.2007.10.029

    Article  PubMed  CAS  Google Scholar 

  13. Tzanavaras PD, Verdoukas A, Balloma T (2006) Optimization and validation of a dissolution test for famotidine tablets using flow injection analysis. J Pharm Biomed Anal 41:437–441 doi:10.1016/j.jpba.2005.12.011

    Article  PubMed  CAS  Google Scholar 

  14. Zarghi A, Shafaati A, Foroutan SM, Khoddam A (2005) A development of a rapid HPLC method for determination of famotidine in human plasma using a monolithic column. J Pharm Biomed Anal 39:677–680 doi:10.1016/j.jpba.2005.03.029

    Article  PubMed  CAS  Google Scholar 

  15. Campanero MA, Bueno I, Arangoa MA, Escolar M, Quetglás EG, López-Ocáriz A, Azanza JR (2001) Improved selectivity in detection of polar basic drugs by liquid chromatography-electrospray ionization mass spectrometry. Illustration using an assay method for the determination of famotidine in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci 763:21–33 doi:10.1016/S0378-4347(01)00355-3

    Article  CAS  Google Scholar 

  16. Zhong L, Eisenhandler R, Yeh KC (2001) Determination of famotidine in low-volume human plasma by normal-phase liquid chromatography/tandem mass spectrometry. J Mass Spectrom 36:736–741 doi:10.1002/jms.176

    Article  PubMed  CAS  Google Scholar 

  17. Simon RE, Walton LK, Liang Y, Denton MB (2001) Fluorescence quenching high-performance thin-layer chromatographic analysis utilizing a scientifically operated charge-coupled device detector. Analyst (Lond) 126:446–450 doi:10.1039/b006799g

    Article  CAS  Google Scholar 

  18. Abdel Kader SA, Abdel Kawy MA, Nebsen M (1999) Spectrophotometric and spectrofluorimetric determination of famotidine and ranitidine using 1,4-benzoquinone. Anal Lett 32:1403–1419 doi:10.1080/00032719908542879

    Article  Google Scholar 

  19. El- Bayoumi A, El- Shanawany AA, El-Sadek ME, Abd-El-Sattar A (1997) Synchronous spectrofluorimetric determination of famotidine, fluconazole and ketoconazole in bulk powder and in pharmaceutical dosage forms. Spectrosc Lett 30:25–46 doi:10.1080/00387019708002587

    Article  Google Scholar 

  20. The British Pharmacopoeia (2008) The Stationary Office, London, p 879

  21. United States Pharmacopoeia XXX: the National Formulary XXV (2008) United States Pharmacopoeial Convention: Rockville, MD, Electronic Version, p 2137

  22. Abdel- Hay MH, Galal SM, Bedair MM, Gazy AA, Wahbi AM (1992) Spectrofluorimetric determination of guanethidine sulphate, guanoxan sulphate and amiloride hydrochloride in tablets and in biological fluids using 9,10-phenanthraquinone. Talanta 39:1369 doi:10.1016/0039-9140(92)80252-9

    Article  PubMed  CAS  Google Scholar 

  23. Belal F, El- Ashry SM, El Kerdawy MM, El Waseef DR (2001) Spectrofluorimetric determination of streptomycin in dosage forms and in spiked plasma using 9,10-phenanthraquinone. J Pharm Biomed Anal 26:435–441 doi:10.1016/S0731-7085(01)00422-8

    Article  PubMed  CAS  Google Scholar 

  24. Guidance for industry; Q2B of analytical procedure: Methodology; International Conference on Hormonization (ICH), November 1996. http://www.fda.gov/eder/guidance/1320fnl

  25. Miller JN, Miller JC (2005) Statistics and chemometrics for analytical chemistry, 5th ed. Prentice Hall, England, p 256

    Google Scholar 

  26. Inczedy J (1976) Analytical and application of complex equilibria. John Wiley and Sons Inc, Buda Pest, p 101

    Google Scholar 

  27. Moffat AC, Osselton MD, Widdop B (2004) Clark’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material, 3rd edn. The Pharmaceutical Press, USA, p 1015

    Google Scholar 

  28. Sawyer DT, Heineman WR, Beebe JM (1984) Chemistry experiments for instrumental methods. Wiley, New York, pp 198–200

    Google Scholar 

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

    M. I. Walash, A. El-Brashy, N. El-Enany & M. E. Kamel

Authors
  1. M. I. Walash
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  2. A. El-Brashy
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  3. N. El-Enany
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  4. M. E. Kamel
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Correspondence to N. El-Enany.

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Walash, M.I., El-Brashy, A., El-Enany, N. et al. Spectrofluorimetric Determination of Famotidine in Pharmaceutical Preparations and Biological Fluids. Application to Stability Studies. J Fluoresc 19, 333–344 (2009). https://doi.org/10.1007/s10895-008-0421-3

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  • DOI: https://doi.org/10.1007/s10895-008-0421-3

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