Abstract
The fluorescence enhancement of the BSA adsorbed on the surface of Ag nanoparticles is reported, where non-fluorescent collagen is used as the separator between the BSA and Ag nanoparticles. The study indicates that Ag nanoparticles can enhance the fluorescence of the BSA, especially the fluorescence of the tyrosine residues with lower quantum. Three types of Ag nanoparticles are evaluated including Ag island film, Ag colloids and fractal Ag electrode. Of them Ag island film is the best. The investigation suggests that the fluorescence enhancement of the BSA is related to the adsorption of the BSA on the surface of Ag island film through the hydrophobic interaction, while the collagen can promote the adsorption of the BSA on the surface of Ag island film and change its conformation, resulting in the interaction between BSA and Ag island film.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Li N, Cook L, Santos C, Cass CE, Mackey JR, Dovichi N (2002) Use of a small reporter molecule to determine cell-surface proteins by capillary electrophoresis and laser-induced fluorescence: use of 5-SAENTA-x8f for quantitation of the human equilibrative nucleoside transporter 1 protein. Anal Chem 74(11):2573–2577 doi:10.1021/ac025559r
Li YF, Shen XW, Huang CZ (2008) A coupled reagent of o-phthalaldehyde and sulfanilic acid for protein detection basedon the measurements of light scattering signalswith a Common spectrofluorometer. Talanta 75:1041–1045 doi:10.1016/j.talanta.2008.01.004
Wu X, Zheng JH, Ding HH, Ran DH, Xu W, Song YY et al (2007) Study on the interaction between oxolinic acid aggregates and protein and its analytical application. Anal Chim Acta 596:16–22 doi:10.1016/j.aca.2007.05.051
Dumitrescu RS (2003) Nitric oxide reduction by heme-thiolate enzymes (P450nor): a reevaluation of the mechanism. Eur J Inorg Chem (6):1048–1052 doi:10.1002/ejic.200390136
Yasui N, Koide T (2003) Collagen–protein interactions mapped by phototriggered thiol introduction. J Am Chem Soc 125(51):15728–15729 doi:10.1021/ja038148g
Kuroda K, Kato M, Mima J, Ueda M (2006) Systems for the detection and analysis of protein–protein interactions. Appl Microbiol Biotechnol 71:127–136 doi:10.1007/s00253-006-0395-5
Ran DH, Wu X, Zheng JH, Yang JH, Zhou HP, Zhang MF et al (2007) Study on the interaction between florasulam and bovine serum albumin. J Fluoresc 17:721–726 doi:10.1007/s10895-007-0226-9
Patiln GV (2003) Biopolymer albumin for diagnosis and in drug delivery. Drug Dev Res 58(3):219–247 doi:10.1002/ddr.10157
George M, Abraham TE (2006) Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan—a review. J Control Release 114:1–14 doi:10.1016/j.jconrel.2006.04.017
Anderson JP, Griffiths M, Boveia VR (2006) Near-infrared fluorescence enhancement using silver island films. Plasmonics 1:103–110 doi:10.1007/s11468-006-9018-3
Stranik O, Nooney R, McDonagh C, MacCraith BD (2007) Optimization of nanoparticle size for plasmonic enhancement of fluorescence. Plasmonics 2:15–22 doi:10.1007/s11468-006-9020-9
Parfenov A, Gryczynski I, Malicka J, Geddes CD, Lakowicz JR (2003) Enhanced fluorescence from fluorophores on fractal silver surfaces. J Phys Chem B 107(34):8829–8833 doi:10.1021/jp022660r
Malicka J, Gryczynski I, Lakowicz JR (2003) DNA hybridization assays using metal-enhanced fluorescence. Biochem Biophys Res Commun 306(1):213–218 doi:10.1016/S0006-291X(03)00935-5
Geddes CD, Lakowicz JR (2002) Editorial: metal-enhanced fluorescence. J Fluoresc 12(2):121–129 doi:10.1023/A:1016875709579
Malicka J, Gryczynski I, Fang JY, Kusba J, Lakowicz JR (2002) Photostability of Cy3 and Cy5-labeled DNA in the presence of metallic silver particles. J Fluoresc 12(3–4):439–447 doi:10.1023/A:1021370111590
Geddes CD, Parfenov A, Gryczynski I, Lakowicz JR (2003) Luminescent blinking from silver nanostructures. J Phys Chem B 107(37):9989–9993 doi:10.1021/jp030290g
Aslan K, Holley P, Geddes CD (2006) Microwave-accelerated metal-enhanced fluorescence (MAMEF) with silver colloids in 96-well plates: application to ultra fast and sensitive immunoassays, high throughput screening and drug discovery. J Immunol Methods 312:137–147 doi:10.1016/j.jim.2006.03.009
Geddes CD, Cao H, Gryczynski I, Gryczynski Z, Lakowicz JR (2006) Metal-enhanced fluorescence (mef) due to silver colloids on a planar surface: potential applications of indocyanine green to in vivo imaging. J Phys Chem A 107(18):3443–3449 doi:10.1021/jp022040q
Lakowicz JR, Shen B, D’Auria S, Malicka J, Fang J, Gryczynski Z et al (2002) Radiative decay engineering: 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer. Anal Biochem 301:261–277 doi:10.1006/abio.2001.5503
Lakowicz JR, Shen B, Gryczynski Z, D’Auria S, Gryczynaltered I (2001) Intrinsic fluorescence from DNA can be enhanced by metallic particles. Biochem Biophys Res Commun 286(5):875–879 doi:10.1006/bbrc.2001.5445
Axelrod D, Burghardt TP, Thompson NL (1984) Total internal reflection fluorescence. Annu Rev Biophys Bioeng 13:247–268 doi:10.1146/annurev.bb.13.060184.001335
Hlady V, Reinecke DR, Andrade JD (1986) Fluorescence of adsorbed protein layers—I. Quantitation of total internal reflection fluorescence. J Colloid Interface Sci 111(2):555–569 doi:10.1016/0021-9797(86)90059-7
Acknowledgments
The Natural Science Foundations of China (20575035) and Shandong Province (Y2003B02) supported this work.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Sun, C., Wu, X., Ding, H. et al. The Fluorescence Enhancement of the Protein Adsorbed on the Surface of Ag Nanoparticle. J Fluoresc 19, 111–117 (2009). https://doi.org/10.1007/s10895-008-0392-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10895-008-0392-4