Journal of Fluorescence

, Volume 18, Issue 2, pp 563–571 | Cite as

An Immunonanogold Resonance Scattering-Quenching Probe for Rapid and Sensitive Assay of Microalbumin

Original Paper

Abstract

A novel and sensitive immunonanogold resonance scattering (RS) spectral probe was obtained for rapid detection of microalbumin (Malb), using 10 nm gold nanaoparticle to label goat anti-human Malb. It was based on that the gold-labeled anti-Malb took place nonspecific aggregation and exhibited a strong RS peak at 577 nm in pH 5.2 C6H8O7–Na2HPO4 buffer solution containing polyethylene glycol (PEG), and the immunocomplex formed after specific reaction of gold-labeled anti-Malb with Malb, which led to a decrease in the intensity of RS peak at 577 nm considerably. The decreased RS intensity at 577 nm (ΔI577nm) was linear to the concentration of Malb in the range of 4–128 ng/mL, with a detection limit of 3.2 ng/mL. The proposed method was applied to detect Malb in healthy human urine samples with satisfactory results.

Keywords

Microalbumin Nanogold-labeled Resonance scattering quenching 

References

  1. 1.
    Bessonova EA, Kartsova LA, Shmukov AU (2006) Electrophoretic determination of albumin in urine using on-line concentration techniques. J Chromatogr A 1150(1–2):332–338PubMedGoogle Scholar
  2. 2.
    Kessler MA, Meinitzer A, Wolfbeis OS (1997) Microalbuminuria and borderline-increased albumin excretion determined with a centrifugal analyzer and the Albumin Blue 580 fluorescence assay. Clin Chem 43:996–1002PubMedGoogle Scholar
  3. 3.
    Khawali C, Andriolo A, Ferreira SRG (2002) Comparison of methods for urinary albumin determination in patients with type 1 diabetes. Braz J Med Biol Res 35(3):337–343PubMedCrossRefGoogle Scholar
  4. 4.
    Pressler BM, Vaden SL, Jensen WA (2002) Detection of canine microalbuminuria using semiquantitative test strips designed for use with human urine. Vet Clin Path 31(2):56–60CrossRefGoogle Scholar
  5. 5.
    Karalliedde J, Viberti G (2004) Microalbuminuria and cardiovascular risk. Am J Hypert 17(10):986–993CrossRefGoogle Scholar
  6. 6.
    Vlachou E, Gosling P, Moiemen NS (2006) Microalbuminuria: a marker of endothelial dysfunction in thermal injury. Burns 32(8):1009–1016PubMedCrossRefGoogle Scholar
  7. 7.
    Erdmann E (2006) Microalbuminuria as a marker of cardiovascular risk in patients with type 2 diabetes. Int J Cardiol 107(12):147–153PubMedCrossRefGoogle Scholar
  8. 8.
    Wada M, Nagasawa H, Kurita K, Koyama S, Arawaka S, Kawanami T, Tajima K, Daimon M, Kato T (2007) Microalbuminuria is a risk factor for cerebral small vessel disease in community-based elderly subjects. J Neurol Sci 255(1–2):27–34PubMedCrossRefGoogle Scholar
  9. 9.
    Zhao LX, Lin JM, Li ZJ (2005) Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine. Anal Chim Acta 541(1–2):197–205CrossRefGoogle Scholar
  10. 10.
    Yu M, Zhu YQ (1991) Clinnical value of joint detection of urine microalbumin, β-2 microglobulin, TH albumen of the patients with diabetes mellitus. Chin J Intern Med 30:354–356Google Scholar
  11. 11.
    Thakkar H, Newman DJ, Holownia P, Davey CL, Wang CC, Lloyd J, Craig AR, Price CP (1997) Development and validation of a particle-enhanced turbidimetric inhibition assay for urine albumin on the Dade aca analyzer. Clin Chem 43:109–113PubMedGoogle Scholar
  12. 12.
    Sviridov D, Meilinger B, Drake SK, Hoehn GT, Hortin GL (2006) Coelution of other proteins with albumin during size-exclusion HPLC: implications for analysis of urinary albumin. Clin Chem 52:389–397PubMedCrossRefGoogle Scholar
  13. 13.
    Contois JH, Hartigan C, Rao LV, Snyder LM, Thompson MJ (2006) Analytical validation of an HPLC assay for urinary albumin. Clin Chim Acta 367(1–2):150–155PubMedCrossRefGoogle Scholar
  14. 14.
    Lu M, Ibraimi F, Kriz D, Kriz K (2006) A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine. Biosen Bioelectron 21(12):2248–2254CrossRefGoogle Scholar
  15. 15.
    Sun Q, Yang GH, Wang H (2004) Clinical evaluation of two determination methods for urinary microalbumin. Lab Immunoass Clin Med 11:235–237CrossRefGoogle Scholar
  16. 16.
    Choi S, Choi EY, Kim HS, Oh SW (2004) On-Site quantification of human urinary albumin by a fluorescence immunoassay. Clin Chem 50:1052–1055PubMedCrossRefGoogle Scholar
  17. 17.
    Qin QP, Peltola O, Pettersson1 K (2003) Time-resolved fluorescence resonance energy transfer assay for point-of-care testing of urinary albumin. Clin Chem 49:1105–1113PubMedCrossRefGoogle Scholar
  18. 18.
    Chan OTM, Herold DA (2006) Chip electrophoresis as a method for quantifying total micro albuminuria. Clin Chem 52:2141–2146PubMedCrossRefGoogle Scholar
  19. 19.
    Jia Z, Yang JH, Wu X, Sun CX, Liu SF, Wang F, Zhao ZS (2006) The sensitive determination of nucleic acids using resonance light scattering quenching method. Spectrochim Acta A 64(3):555–559CrossRefGoogle Scholar
  20. 20.
    Liu SP, Luo HQ, Li NB, Liu ZF, Zheng WX (2001) Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes. Anal Chem 73(16):3907–3914PubMedCrossRefGoogle Scholar
  21. 21.
    Chen ZG, Liu JB, Han YL (2007) Rapid and sensitive determination of proteins by enhanced resonance light scattering spectroscopy of sodium lauroyl glutamate. Talanta 71(3):1246–1251CrossRefPubMedGoogle Scholar
  22. 22.
    Bao P, Frutos AG, Greef C, Lahiri J, Muller U, Peterson TC, Warden L, Xie XY (2002) High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering. Anal Chem 74(8):1792–1797PubMedCrossRefGoogle Scholar
  23. 23.
    Xi ZG (2004) Nano-technology application in environmental sanitation. Chemical Industry Press, Beijing, pp 24–40Google Scholar
  24. 24.
    Jiang ZL, Sun SJ, Liang AH, Huang WX, Qin AM (2006) Gold-labeled nanoparticle-based immunoresonance scattering spectral assay for trace apolipoprotein AI and apolipoprotein B. Clin Chem 52:1389–1394PubMedCrossRefGoogle Scholar
  25. 25.
    Jiang ZL, Sun SJ, Liang AH (2006) A new immune resonance scattering spectral assay for trace fibrinogen with gold nanoparticle label. Anal Chim Acta 571(2):200–205PubMedCrossRefGoogle Scholar
  26. 26.
    Hou M, Sun SJ, Jiang ZL (2007) A new and selective and sensitive nanogold-labeled immunoresonance scattering spectral assay for trace prealbumin. Talanta 72(2):463–467CrossRefPubMedGoogle Scholar
  27. 27.
    Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspension. Nat Phys Sci 241:20–22Google Scholar
  28. 28.
    Chen XF, Liu SZ (2004) Colloidal gold labeled immunoassay and it’s application in rapid detection of small molecule. Pharm Biotechnol 11:278–280Google Scholar
  29. 29.
    Oliver C (1999) Conjugation of colloidal gold to proteins. Method Mol Biol 115(3):331–334Google Scholar
  30. 30.
    Su YL (2006) A strategy for immunoassay signal amplification using clusters of immunogold nanoparticles. Appl Surf Sci 253(3):1101–1106CrossRefGoogle Scholar
  31. 31.
    Steffen W, Hodgkinson JL, Wiche G (1996) Immunogold localisation of the intermediate chain within the protein complex of cytoplasmic dynein. J Struct Biol 117(3):227–235PubMedCrossRefGoogle Scholar
  32. 32.
    Wang H, Zhen ZY, Zeng RY, Zhu DH, Tao YX (1996) ELISA for albumin in urine by monoclonal antibody. Shanghai J Med Lab Sci 11:17–18Google Scholar
  33. 33.
    Liang AH, Huang YJ, Jiang ZL (2007) A rapid and sensitive immunoresonance scattering spectral assay for microalbumin. Clin Chim Acta 383(1–2):73–77PubMedCrossRefGoogle Scholar
  34. 34.
    Mueller PW, MacNeil ML, Smith SJ, Miller DT (1991) Interlaboratory comparison of the measurement of albumin in urine. Clin Chem 37:191–195PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.School of Environment and ResourceGuangxi Normal UniversityGuilinChina
  2. 2.Department of Material and Chemical EngineeringGuilin University of TechnologyGuilinChina

Personalised recommendations