Journal of Fluorescence

, Volume 18, Issue 2, pp 563–571

An Immunonanogold Resonance Scattering-Quenching Probe for Rapid and Sensitive Assay of Microalbumin

Original Paper

DOI: 10.1007/s10895-007-0300-3

Cite this article as:
Jiang, ZL., Huang, YJ. & Liang, AH. J Fluoresc (2008) 18: 563. doi:10.1007/s10895-007-0300-3


A novel and sensitive immunonanogold resonance scattering (RS) spectral probe was obtained for rapid detection of microalbumin (Malb), using 10 nm gold nanaoparticle to label goat anti-human Malb. It was based on that the gold-labeled anti-Malb took place nonspecific aggregation and exhibited a strong RS peak at 577 nm in pH 5.2 C6H8O7–Na2HPO4 buffer solution containing polyethylene glycol (PEG), and the immunocomplex formed after specific reaction of gold-labeled anti-Malb with Malb, which led to a decrease in the intensity of RS peak at 577 nm considerably. The decreased RS intensity at 577 nm (ΔI577nm) was linear to the concentration of Malb in the range of 4–128 ng/mL, with a detection limit of 3.2 ng/mL. The proposed method was applied to detect Malb in healthy human urine samples with satisfactory results.


Microalbumin Nanogold-labeled Resonance scattering quenching 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.School of Environment and ResourceGuangxi Normal UniversityGuilinChina
  2. 2.Department of Material and Chemical EngineeringGuilin University of TechnologyGuilinChina

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