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Study of the Interaction Between Apis mellifera Venom and Micro-Heterogeneous Systems

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The bee venom, used in treatment of inflammatory and articular diseases, is a complex mixture of peptides and enzymes and the presence of tryptophan allows the investigation by fluorescence techniques. Steady state and time-resolved fluorescence spectroscopy were used to study the interaction between bee venom extracted from Apis mellifera and three micro heterogeneous systems: sodium dodecylsulphate (SDS) micelles, sodium dodecylsulphate-poly(ethylene oxide) (SDS-PEO) aggregates, and the polymeric micelles LUTROL® F127, formed by poly(ethylene oxide)-poly(propylene oxide)- poly(ethylene oxide). Fluorescence parameters in buffer solution were typical of peptides containing tryptophan exposed to the aqueous medium, and they gradually changed upon the addition of surfactant and polymeric micelles, demonstrating the interaction of the peptides with the micro heterogeneous systems. Quenching experiments were carried out using the N-alkylpyridinium ions (ethyl, hexyl, and dodecyl) as quenchers. In buffer solution the quenching has low efficiency and is independent of the alkyl chain length of the quencher. In the presence of the micro heterogeneous systems the extent of static and dynamic quenching enhanced, showing that both fluorophore and quenchers reside in the microvolume of the aggregates. The more hydrophobic quencher (dodecyl pyridinium ion) provides higher values for K SV and dynamic quenching constants, and SDS-PEO aggregates are most efficient to promote interaction between peptides and alkyl pyridinium ions. The results proved that bee venon interacts with drug delivery micelles of the copolymer LUTROL® F127.

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Acknowledgements

This work was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—Proc. 03/05878-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. The authors are pleased to thank Prof. Dr. Antonio Claudio Tedesco from Departamento de Química da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo for the use of the spectrofluorometer Hitachi F4500 and Dr. Carla Cristina Schmitt Cavalheiro from Instituto de Química de São Carlos, Universidade de São Paulo, for the support in light scattering measurements.

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Authors and Affiliations

  1. Departamento de Física e Matemática, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Ana Paula Romani, Cássia Alessandra Marquezin & Amando Siuiti Ito

  2. Departamento de Genética da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Ademilson Espencer Egea Soares

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  1. Ana Paula Romani
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  2. Cássia Alessandra Marquezin
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  3. Ademilson Espencer Egea Soares
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  4. Amando Siuiti Ito
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Correspondence to Amando Siuiti Ito.

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Romani, A.P., Marquezin, C.A., Soares, A.E.E. et al. Study of the Interaction Between Apis mellifera Venom and Micro-Heterogeneous Systems. J Fluoresc 16, 423–430 (2006). https://doi.org/10.1007/s10895-006-0077-9

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  • DOI: https://doi.org/10.1007/s10895-006-0077-9

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