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A Sensitive Fluorimetric Method for the Determination of Epinephrine

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Abstract

A sensitive fluorimetric method for the determination of epinephrine (E) is described in this paper. The experiments indicate that epinephrine can react with formaldehyde (HCHO) in an acid medium to form a condensation product, which can be oxidized by potassium hexacyanoferrate(III) (K3[Fe(CN)6]) in borax buffer (pH = 9.5). The reaction product can emit strong fluorescence. Ascorbic acid (AA) is used in order to consume excess potassium hexacyanoferrate and stabilize the fluorescent product. Under optimum conditions, a linear relationship has been obtained between the fluorescence intensity and the concentration of epinephrine in the range of 1.4×10−9−2.1×10−6 mol/l, and the detection limit is 2.4×10−10 mol/l (4.3×10−11 g/ml, S/N = 3). The method is applied for the determination of E in both actual sample and the synthetic sample with E and norepinephrine (NE) by using the coupling technique of synchronous fluorimetry and H-point standard addition method, and the results obtained are satisfactory.

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Authors and Affiliations

  1. Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People’s Republic of China

    Yumei Guo, Jinghe Yang, Xia Wu & Aiqin Du

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  1. Yumei Guo
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  2. Jinghe Yang
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  3. Xia Wu
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  4. Aiqin Du
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Correspondence to Jinghe Yang.

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Guo, Y., Yang, J., Wu, X. et al. A Sensitive Fluorimetric Method for the Determination of Epinephrine. J Fluoresc 15, 131–136 (2005). https://doi.org/10.1007/s10895-005-2520-8

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  • DOI: https://doi.org/10.1007/s10895-005-2520-8

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