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Reaction of Individual Physiological Barriers in Bacterial Infection in Different Races of the Honeybee Apis mellifera

  • Comparative and Ontogenic Physiology
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Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript


This work deals with study of differences between races of the honeybee Apis mellifera with respect to involvement in the protective reaction to bacterial infection of the barrier physiological mechanisms—of responses of intestine and hemolymph cells. The states of the intestine parts is evaluated; the rates of accumulation and elimination of bacteria from the midgut are determined in the honeybees treated once and repeatedly by a preparation based on the food Bacillus thuringiensis (BTB). The hemolymph cells were also studied cytologically. It was shown that under conditions of this experiment the honeybees of the middle-Russian race were characterized by an optimal combination and participation of all considered barrier mechanisms in response to penetration into the organism and development of the bacterial pathogen. A suggestion is put forward about the genetically fixed differences in functions of the physiological mechanisms between the diverged races as well as about the incomplete realization of adaptive potential in the honeybees of Caucasian race introduced in the Republic and in hybrid individuals due to the stress produced by the environmental factors.

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  1. Kuznetsov, N.Ya., Osnovy fiziologii nasekomykh (Foundations of Insect Physiology), Leningrad, 1948, vol. 1, pp. 124–131.

    Google Scholar 

  2. Bai, C., Vanhaecke, M., and Degheele, D., Cytopathology of Spodoptera littoralis Boisd. Midgut Epithelium following Treatment with β-Endotoxin of Bacillus thuringiensis Berliner, Meded. Fac. Landbouwwetensch. Rijksuniv. Gent, 1984, vol. 49, pp. 875–884.

    Google Scholar 

  3. Kuzmanova, I., Tersiiski, D., and Karageorgiev, S., Electron Microsopic Study of Histopathological Changes of Gastric Epithelium in Larvae of Leptinotarsa decemlineata Say Treated with Preparation Containing β-Exotoxin of Bacillus thuringiensis, Nats. Konf. po entomol. (Nat. Conf. on Entomol.), Sophia, 1991, pp. 46–51.

  4. Tielecke, H., Der Einflß Subletaler Dosen on Insektiziden auf die Biologishen Daten und auf die Resistenz Bildung Einiger Insekten, Arch. Phytopathol. Pflanzenschutz, 1997, vol. 13, pp. 277–288.

    Google Scholar 

  5. Czajka, M.C. and Lee, R.E., A Rapid Cold Hardening Response Protecting against Cold Shock Injury in Drosophila melanogaster, J. Exp.Biol., 1990, vol. 148, pp. 245–254.

    PubMed  Google Scholar 

  6. Yocum, G.D. and Denlinger, D.L., Anoxia Blocks Thermotolerance and the Induction of Rapid Cold Hardening in the Flesh Fly, Sarcophaga crassipalpis, Physiol. Entomol., 1994, vol. 19, pp. 152–158.

    Google Scholar 

  7. Watson, M.J.O. and Hoffman, A.A., Acclimation, Cross-Generation Effects, and the Response to Selection for Increased Cold Resistance in Drosophila, Evolution (USA), 1996, vol.50, pp. 1182–1192.

    Google Scholar 

  8. Valyukas, Yu.B., Zayanchkauskas, P.A., and Babyanskas, M.A., Effect of Immune Serum and Entomopathogenic Bacteria on Resistance in Insects, Noveishie dostizheniya selskokhozyaistvennoi entomologii, Mat. VII s”Iezda VEO (Recent Achievements of Agricultural Entomology, Proc. VII Conf. UES), Vilnyus, 1981, pp. 27–31.

  9. Bartinkaite, I.S., Effect of the Entobacterin Antigen on Resistance to Entobacterin in Insects, Tr. Akad. Nauk LitSSR, 1987, B, No.2, pp. 63–71.

    Google Scholar 

  10. Saltykova, E.S., Poskryakov, A.V., Nikolenko, A.G., and Khairullin, R.M., Increase of Adaptivity of the Honeybee by Using Chitooligosaccharides, Ekologicheskii imperativ sel’skogo khozyaistva Respubliki Bashkortostan (Ecologic Imperative of Agriculture of the Bashkortostan Republic), Ufa, 1998, pp. 67–68.

  11. Saltykova, E.S., Adaptive Effect of Chitooligosaccharides on Apis melifera, Cand. Sci. Dissertation, St. Petersburg, 2000.

  12. Spravochnik po Klinicheskim Laboratornym Metodam (Reference Book of Clinical Laboratory Methods), Moscow, 1957

  13. Zapolskikh, O.V., Comparative-Morphological and Cytochemical Study of Hemolymph Cells of Some Hymenoptera, Cand. Sci. Dissertation, Leningrad, 1978.

  14. Lakin, G.F., Biometriya (Biometria), Moscow, 1990.

  15. Kandybin, N.V., Bakterialnye sredstva bor’by s gryzunami i vrednymi nasekomymi (Bacterial Preparations for the Fight with Rodents and Destructive Insects), Moscow, 1989.

  16. Baturin, V.V. and Baturina, L.I., Peculiarities of the Infectious Process in Lepidopterous and Orthopterous Insects during Their Infestation with Crystallo-form Bacteria of the Thuriengiensis Group, Mikroorganismy v zashchite rastenii ot vrednykh nasekomykh (Microorganisms in the Plant Protection from Destructive Insects), Irkutsk, 1978, pp. 97–108.

  17. Sikura, A.I., On Muscardin Infection in Larvae of the American Cabbage White Butterfly, Dop. Akad. Nauk UkrSSR, 1957, p. 598.

  18. Primak, T.A., The Blood Picture in Representatives of the Pieridae Family in Diseases and Infestation with Parasites, Biologicheskii metod bor’by s vreditelyami rastenii (Biological Method of Fight with Pests of Plants), Kiev, 1959, pp. 106–112.

  19. Shevkunov, V.S., Phagocytic Reactions and Bactericidal Action of Insect Hemolymph, Izv. SO AN SSSR, Ser.Biol.-Med. Nauk, 1968, issue 1, pp. 90–94.

  20. Zengbush, P., Molekulyarnaya i kletochnaya biologiya (Molecular and Cell Biology), Moscow, 1982, vol. 2.

  21. Lai-Fook, J., The Structure of the Hemocytes of Calpode sethlius (Lepidoptera), J. Morphol., 1973, vol. 139, pp. 9–86.

    Article  Google Scholar 

  22. Neuwirth, M., The Structure of the Hemocytes of Galleria mellonella (Lepidoptera), J. Morphol., 1973, vol. 139, pp. 105–113.

    Article  Google Scholar 

  23. Ashhurs, D.E. and Glenn, R.A., Some Histochemical Observations on the Blood Cells of the Wax Moth, Galleria mellonella L., J. Morphol., 1964, vol. 114, pp. 247–253.

    Article  PubMed  Google Scholar 

  24. Miselyunene, I., Changes of Morphology and Ratio of Various Types of Hemolymph Cells in Cabbage Pierids Infestated with Entobacterin, Tsitologiya, 1976, vol. 18, pp. 1220–1225.

    Google Scholar 

  25. Otskheli, T.A., Study of Hemolymph of the Silkworm Larvae under Conditions of the Changed Nutrition Regime, Tr. Inst. Zool. AN GruzSSR, 1954, pp. 215–222.

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Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 3, 2005, pp. 254–258.

Original Russian Text Copyright © 2005 by Saltykova, Ben’kovskaya, Gaifullina, Novitskaya, Poskryakov, Nikolenko.

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Saltykova, E.S., Ben’kovskaya, G.V., Gaifullina, L.R. et al. Reaction of Individual Physiological Barriers in Bacterial Infection in Different Races of the Honeybee Apis mellifera . J Evol Biochem Phys 41, 318–324 (2005).

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