Abstract
Effects were studied of single (40 min) or repeated exercise load (tredmill running, 14 m/min, 30 min) on the physiological and biochemical blood parameters characterizing the organism energetic and metabolic processes in complex experiments on male Wistar rats, on spontaneously hypertensive rats of the SHR line, and their normotensive control WKY. The mixed blood was used for determination of hematocrit, red blood cell count, hemoglobin concentration, level of 2,3-diphosphoglycerate (2,3-DPG) in red blood cells, erythrocyte acetylcholinesterase (AChE) activity, glucose, cholesterol, and triglyceride concentration, as well as plasma corticosterone level. To assess the erythrocyte population characteristics, the acidity erythrogram was determined. The weights of adrenal glands and spleen were evaluated. The single running induced a typical stress-response. After the repeated exercise load (the 7–14-day running), Wistar and WKY rats were well adopted to the load, unlike the SHR line rats that were practically not adapted to the repeated tredmill running.
Similar content being viewed by others
REFERENCES
Selie, G., Ocherki ob adaptatsionnom sindrome (Essays on Adaptation Syndrome), Moscow, 1960.
Meerson, F.Z., Adaptatsiya, stress i profilaktika (Adaptation, Stress, and Prevention), Moscow, 1981.
Meerson, F.Z., Adaptation to Stress Situations and Stress-Limiting Systems, Fiziologiya adaptatsionnykh protsessov (Physiology of Adaptation Processes), Moscow, 1986, pp. 521–531.
Sudakov, K.V., Stress: Postulates, Analysis from the Viewpoint of General Theory of Functional Systems, Patol. Fiziol. Eksp.Terap., 1992, no. 4, pp. 86–93.
Malyshev, I.Yu. and Manukhina, E.B., Stress, Adaptation, and Nitrogen Oxide, Biokhimiya, 1998, vol. 63, pp. 992–1000.
Medvedeva, I.A. and Maslova, M.N., Dynamics and Mechanism of Change of Activity of Rat Erythrocyte Na,K-ATPase during Action of Stressors of Different Etiology, Sechenov Fisiol. Zh., 1993, vol. 79, no.12, pp. 28–34.
Gitelzon, I.I. and Terskov, I.A., Eritrogrammy kak metod klinicheskogo issledovaniya krovi (Erythrograms as a Method of Clinical Study of Blood), Krasnoyarsk, 1959.
Maslova, M.N. and Reznik, L.V., Inhibition of Cholinesterase Activity of Rat Brain by Phosphororganic Inhibitors with Different Degree of Hydrophobity, Ukr. Biokh. Zh., 1976, vol. 48, pp. 450–454.
Kazennov, A.M., Maslova, M.N., and Shalabodov, A.D., Study of Na,K-ATPase Activity in Mammalian Erythrocytes, Biokhimiya, 1984, vol. 49, pp. 1098–1095.
Balashov, I.G., Fluorimetric Micromethod for Determination of Corticosteroids: Comparison of Three Methods, Fiziol. Zh. SSSR, 1990, vol. 76, pp. 280–283.
Fowles, J.R., Green, H.J., Schertzer, J.D., and Tupling, A.R., Reduced Activity of Muscle Na(+),K(+)-ATPase after Prolonged Running in Rats, J. Appl. Physiol., 2002, vol. 93, pp. 1703–1708.
Meen, H.D., Holter, P.H., and Refsum, H.E., Changes in 2,3-Diphosphoglycerate (2,3-DPG) after Exercise, Eur. J. Appl. Physiol., 1981, vol. 46, pp.177–184.
Maslova, M.N., Activity of Membrane Enzymes of Erythrocytes at Various Stress Actions, Sechenov Fiziol. Zh., 1994, vol. 80, no.7, pp. 78–80.
Katyukhin, L.N. and Maslova, M.N., Characterization of Blood Erythrocytes in Essential Hypertension, Lab. Delo, 1989, vol. 61, no.11, pp. 49–51.
Kazennov, A.M., Maslova, M.N., Gurevich, V.S., Bagrov, J.Yu., and Shalabodov, A.D., Some Properties of Erythrocyte Na,K-ATPase in Essential Hypertension, Clin. Exp. Hypertension, 1987, vol. 9A, no.7, pp. 121–132.
Kim, S.H., Kim, H.B., Jang, M.H., Lim, B.V., Kim, Y.J., Kim, J.P., Kim, S.S., Kim, E.H., and Kim, C.J., Tredmill Exercise Increases Cell Proliferation without Altering of Apoptosis in Dentat Gyrus of Sprague-Dawley Rats, Life Sci., 2002, vol. 71, pp.1331–1340.
Timofeeva, E., Huang, Q., and Richard, D., Effects of Tredmill Running on Brain Activation and the Corticotropin-Releasing Hormone System, Neuroendocrinol., 2003, vol. 72, pp. 388–405.
Author information
Authors and Affiliations
Additional information
__________
Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 2, 2005, pp. 129-133.
Original Russian Text Copyright © 2005 by Maslova, Khama-Murad, Kazennov, Kislyakova, Tavrovskaya, Barvitenko.
Rights and permissions
About this article
Cite this article
Maslova, M.N., Khama-Murad, A.L., Kazennov, A.M. et al. Changes of Functional and Biochemical Blood Parameters in Wistar Rats and in Spontaneously Hypertensive Rats of the SHR Line during Short-Term and Long Tredmill Running. J Evol Biochem Phys 41, 162–168 (2005). https://doi.org/10.1007/s10893-005-0050-2
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10893-005-0050-2