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Metabolomic Profiling of Phloem Sap from Different Pine Species and Implications on Black Capuchin

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Abstract

In most commercial pine farms in southern Brazil, black capuchin causes damage to wood and financial losses when it removes bark from some pine species to feed upon underlying vascular tissues. Therefore, this study aimed to evaluate the variability of the primary metabolites of phloem saps from 10 different species of pine by NMR spectroscopy, as well as the aroma compounds using SPME–GC–MS. Each technique provided a different set of metabolites that we can correlate to monkey predilection. The PCA showed monosaccharide (detected by NMR) and α-pinene (pine-like and resinous flavor descriptors) as attractive compounds for monkeys. On the other hand, the low content of monosaccharide and the high content of β-phellandrene (citrus odor descriptor) was observed in less attacked pine species (P. patula). The data fusion on primary metabolites and aroma compounds corroborated the individual analyses, complementing the comprehension of the monkey predilection. Thus, P. elliottii was an avoided tree even with high content of sugars possibly due to its high content of β-phellandrene (citrus odor). The results are useful for further behavioral studies to determine the role that each highlighted metabolite plays in chemically mediated animal-plant interactions.

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Acknowledgements

The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnologico (FUNCAP) for scholarship NT-00159-00006.01.00/19 and Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES) for financial support.

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Authors and Affiliations

  1. Embrapa Agroindústria Tropical, Rua Doutora Sara Mesquita, 2270-Pici, Fortaleza, CE, CEP 60511-110, Brazil

    Lorena Mara A. Silva, Guilherme J. Zocolo, Kirley M. Canuto & Edy S. De Brito

  2. Department of Food Engineering, Universidade Federal Do Ceará, Campus do Pici, Bloco 858, Fortaleza, CE, CEP 60440-900, Brazil

    Elenilson G. Alves Filho & Francisca Jamila C. Louredo

  3. Universidade Estadual Do Vale Do Acaraú, Campus da Betânia, Sobral, CE, 62040-370, Brazil

    Tigressa Helena S. Rodrigues

  4. Embrapa Floresta, Estrada da Ribeira, Km 111, Bairro Guaraituba, Colombo, PR, CEP 83411-000, Brazil

    Sandra B. Mikich

  5. Arauka Ambiental, Rua Ten. Ricardo Kirch, 188, Curitiba, Paraná, CEP 81530120, Brazil

    Dieter Liebsch

  6. Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo 2265, São José Do Rio Preto, SP, 15054-000, Brazil

    Adriana De Almeida

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  1. Lorena Mara A. Silva
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  2. Elenilson G. Alves Filho
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  3. Tigressa Helena S. Rodrigues
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  4. Francisca Jamila C. Louredo
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  5. Guilherme J. Zocolo
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  6. Kirley M. Canuto
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  7. Sandra B. Mikich
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  8. Dieter Liebsch
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  9. Adriana De Almeida
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  10. Edy S. De Brito
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Correspondence to Lorena Mara A. Silva.

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Silva, L.M.A., Filho, E.G.A., Rodrigues, T.H.S. et al. Metabolomic Profiling of Phloem Sap from Different Pine Species and Implications on Black Capuchin. J Chem Ecol 48, 660–669 (2022). https://doi.org/10.1007/s10886-022-01365-3

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  • DOI: https://doi.org/10.1007/s10886-022-01365-3

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