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Insights into the Interaction between the Monophagous Tephritid Fly Anastrepha acris and its Highly Toxic Host Hippomane mancinella (Euphorbiaceae)

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Abstract

Despite their enormous economic importance and the fact that there are almost 5000 tephritid (Diptera) species, fruit fly – host plant interactions are poorly understood from a chemical perspective. We analyzed the interactions among Anastrepha acris (a little studied monophagous tephritid) and its highly toxic host plant Hippomane mancinella from chemical, ecological and experimental perspectives, and also searched for toxicants from H. mancinella in the larval-pupal endoparasitoid Doryctobracon areolatus. We identified 18 phenolic compounds from H. mancinella pulp belonging to different chemical groups including phenylpropanoids, flavonoids, chalcones and coumarins. No traces of Hippomanin A were detected in larvae, pupae or A. acris adults, or in D. areolatus adults, implying that A. acris larvae can metabolize this toxicant, that as a result does not reach the third trophic level. We tested the “behavioral preference – lack of larval specialization-hypothesis” via feeding experiments with a larval rearing medium containing H. mancinella fruit (skin + pulp or pulp alone). The high toxicity of H. mancinella was confirmed as only two (out of 2520 in three experiments) A. ludens larvae (a polyphagous pest species that preferentially feeds on plants within the Rutaceae) survived without reaching the adult stage when fed on media containing H. mancinella, whereas A. acris larvae developed well and produced healthy adults. Together, these findings open a window of opportunity to study the detoxification mechanisms used by tephritid fruit flies.

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Acknowledgements

We are grateful to Nicole M. van Dam, Associate Editor of the Journal of Chemical Ecology who handled the initial part of the refereeing process of this manuscript providing many important suggestions for improvement and also shared links containing practical advice on scientific manuscript writing. The final part of the refereeing process was handled by Editor-in-Chief, Gary W. Felton, and we also thank him for many valuable suggestions for improvement and for seeking the opinion of two additional referees. We gratefully acknowledge the important contributions of six anonymous referees, who provided many specific suggestions for improvement and the meticulous revision by the native English speaker Dr. Trevor Williams, who significantly improved the usage of the foreign language. We thank Gabriel A. Hernández-Velázquez, Adriana Santos-Ramiro and Uriel Gallardo Valencia (INECOL) for technical assistance. This work was partially financially by the Asociación de Productores y Empacadores Exportadores de Aguacate de México (Project APEAM-INECOL 41010 to M.A.). Additional funding was provided by the Mexican Campaña Nacional contra Moscas de la Fruta (DGSV-SENASICA-SAGARPA) through the Consejo Nacional Consultivo Fitosanitario (CONACOFI), the MIT-Veracruz Fund, (both to M.A), and the Instituto de Ecología, A.C. (INECOL).

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  1. Instituto de Ecología, A.C. - INECOL, Red de Manejo Biorracional de Plagas y Vectores, Clúster Científico y Tecnológico BioMimic®, Carretera antigua a Coatepec 351, 91073, Xalapa, Veracruz, Mexico

    Martín Aluja, Carlos Pascacio-Villafán, Alma Altúzar-Molina, Erick Enciso, Rafael Ortega, Emilio Acosta & Larissa Guillén

  2. Instituto de Ecología, A.C. – INECOL, Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, Carretera antigua a Coatepec 351, 91073, Xalapa, Veracruz, Mexico

    Juan Monribot-Villanueva & José A. Guerrero-Analco

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  1. Martín Aluja
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  2. Carlos Pascacio-Villafán
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Correspondence to Martín Aluja.

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Aluja, M., Pascacio-Villafán, C., Altúzar-Molina, A. et al. Insights into the Interaction between the Monophagous Tephritid Fly Anastrepha acris and its Highly Toxic Host Hippomane mancinella (Euphorbiaceae). J Chem Ecol 46, 430–441 (2020). https://doi.org/10.1007/s10886-020-01164-8

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