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Molecular Screening of Behaviorally Active Compounds with CmedOBP14 from the Rice Leaf Folder Cnaphalocrocis medinalis

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Abstract

Odorant binding proteins (OBPs) play a key role in chemoreception in insects. In an earlier study, we identified CmedOBP14 from the rice leaf folder, Cnaphalocrocis medinalis, with potential physiological functions in olfaction. Here, we performed a competitive binding assay under different pH conditions as well as knockdown via RNA interference to determine the specific role of CmedOBP14 in C. medinalis. CmedOBP14 displayed broad binding affinities to many host-related compounds, with higher affinities at pH 7.4 compared with pH 5.0. After treatment with CmedOBP14-dsRNA, the transcript level of OBP14 was significantly decreased at 72 h compared with controls, and the electroantennogram response evoked by nerolidol, L-limonene and beta-ionone was reduced. Furthermore, behavioral assays revealed consistent patterns among these compounds, especially for nerolidol, with adults could no longer able to differentiate 0.1% nerolidol from controls. RNAi experiments suggest that at least in part, CmedOBP14 mediates the ability to smell nerolidol and beta-ionone.

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Acknowledgments

This study was supported and funded by the National Key Research and Development Program of China (2017YFD0200400) and the Special Technical Innovation of Hubei Province (2017ABA146).

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  1. Shuang-Feng Sun and Fang-Fang Zeng contributed equally to this work

Authors and Affiliations

  1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Shuang-Feng Sun, Shan-Cheng Yi & Man-Qun Wang

  2. School of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001, People’s Republic of China

    Fang-Fang Zeng

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  1. Shuang-Feng Sun
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Correspondence to Man-Qun Wang.

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Sun, SF., Zeng, FF., Yi, SC. et al. Molecular Screening of Behaviorally Active Compounds with CmedOBP14 from the Rice Leaf Folder Cnaphalocrocis medinalis. J Chem Ecol 45, 849–857 (2019). https://doi.org/10.1007/s10886-019-01106-z

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