Isopentyl Butanoate: Aggregation Pheromone of the Brown Spiny Bug, Clavigralla tomentosicollis (Hemiptera: Coreidae), and Kairomone for the Egg Parasitoid Gryon sp. (Hymenoptera: Scelionidae)

Abstract

The brown spiny bug, Clavigralla tomentosicollis Stål (Hemiptera: Coreidae) is a key pest of leguminous crops in many countries in Africa, causing significant yield losses especially in cowpea, pigeon pea and common beans. Although C. tomentosicollis uses olfaction to aggregate, little is known about the identity of the aggregation pheromone. This study aimed to identify the aggregation pheromone of C. tomentosicollis and to test its potential role in the behavior of its egg parasitoid, Gryon sp. In Y-tube olfactometer bioassays, only male volatiles strongly attracted both sexes of C. tomentosicollis. Coupled gas chromatography/electroantennographic detection (GC/EAD) and GC/mass spectrometry were used to identify antennally-active compounds from male volatiles. Antennae of both sexes detected identical components including a male-specific component, identified as isopentyl butanoate, which was also detected by antenna of the egg parasitoid. In olfactometer bioassays, both sexes of C. tomentosicollis and the egg parasitoid responded to isopentyl butanoate. These results suggest that isopentyl butanoate serves as an aggregation pheromone for both sexes of C. tomentosicollis and a useful kairomone to attract the parasitoid in the management of C. tomentosicollis.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Addesso KM, Mcauslane HJ, Cherry R (2012) Aggregation behavior of the southern chinch bug (Hemiptera: Blissidae). Environ Entomol 41:887–895

    Article  Google Scholar 

  2. Aldrich JR (1975) Natural products of abdominal and metathoracic scent glands of coreid bugs. Ann Entomol Soc Am 68:955–960

    Article  CAS  Google Scholar 

  3. Aldrich JR, Zhang A (2002) Kairomone strains of Euclytia flava (Townsend), a parasitoid of stink bugs. J Chem Ecol 28:1565–1582

    Article  CAS  PubMed  Google Scholar 

  4. Aliyu M, Ladan T, Ahmed BI, Abdullahi J (2007) Studies on the efficacy of black soap and kerosene mixture on the control of pod sucking bugs (Clavigralla tomentosicollis Stål) on cowpea (Vigna unguiculata (L.) Walp). Emir J Food Agric 19:8–14

    Article  Google Scholar 

  5. Asante SK, Jackai LEN, Tamò M (2000) Efficiency of Gryon fulviventris (Hymenoptera : Scelionidae) as an egg parasitoid of Clavigralla tomentosicollis ( Hemiptera : Coreidae ) in northern Nigeria. Environ Entomol 29:815–821

    Article  Google Scholar 

  6. Audino PG, Alzogaray RA, Vassena C, Masuh H, Fontan A, Gatti P, Martinez A, Camps F, Cork A, Zerba E (2007) Volatile compounds secreted by brindley’s glands of adult Triatoma infestans: identification and biological activity of previously unidentified compounds. J Vector Ecol 32:75–82

    Article  Google Scholar 

  7. Conti E, Salerno G, Bin F, Williams HJ, Vinson SB (2003) Chemical cues from Murgantia histrionica eliciting host location and recognition in the egg parasitoid Trissolcus brochymenae. J Chem Ecol 29:115–130

    Article  CAS  PubMed  Google Scholar 

  8. Dabire CLB, Kini FB, Ba MN, Dabire RA, Fouabi K (2005) Effet du stade de développement des gousses de niébé sur la biologie de la punaise suceuse Clavigralla tomentosicollis (Hemiptera: Coreidae). Int J Trop Insect Sci 25:25–31

    Article  Google Scholar 

  9. Dialoke SA, Agu CM, Ojiako FO, Onweremadu E, Onyishi GO, Ozor N, Echezona BC, Ofor MO, Ibeawuchi II, Chigbundu IN, Ngwuta AA, Ugwoke FO (2010) Survey of insect pests on pigeon pea in Nigeria. J SAT Agric Res 8:1–8

    Google Scholar 

  10. Dreyer H, Baumgärtner J (1996) Temperature influence on cohort parameters and demographic characteristics of the two cowpea coreids Clavigralla tomentosicollis and C. shadabi. Entomol Exp Appl 78:201–213

    Article  Google Scholar 

  11. Dreyer H, Baumgärtner J, Tamo M (1994) Seed-damaging field pests of cowpea (Vigna unguiculata L. Walp.) in Benin: occurrence and pest status. Int J Pest Manag 40:252–260

    Article  Google Scholar 

  12. Dzemo WD, Asiwe ASNJ (2010) A comparative study of the bionomics of Clavigralla tomentosicollis Stål (Hemiptera: Coreidae) on three varieties of cowpea (Vigna unguiculata (L.) Walp). Afr J Agric Res 5:567–572

    Google Scholar 

  13. Egwuatu RI, Taylor TA (1976) Aspects of the spatial distribution of Acanthomia tomentosicollis Stål (Heteroptera, Coreidae) in Cajanus cajan (pigeon pea). J Econ Entomol 69:591–594

    Article  Google Scholar 

  14. Egwuatu RI, Taylor TA (1977) Studies on the biology of Acanthomia tomentosicollis (Stål) (Hemiptera: Coreidae) in the field and insectary. Bull Entomol Res 67:249–257

    Article  Google Scholar 

  15. Fatouros NE, Dicke M, Mumm R, Meiners T, Hilker M (2008) Foraging behavior of egg parasitoids exploiting chemical information. Behav Ecol 19:677–689

    Article  Google Scholar 

  16. Fávaro CF, Millar JG, Zarbin PH (2015) Identification and synthesis of the male-produced sex pheromone of the stink bug, Pellaea stictica. J Chem Ecol 41:859–868

    Article  CAS  PubMed  Google Scholar 

  17. Guerenstein PG, Guerin PM (2004) A comparison of volatiles emitted by adults of three Triatomine species. Entomol Exp Appl 111:151–155

    Article  CAS  Google Scholar 

  18. James DG, Moore CJ, Aldrich JR (1994) Identification, synthesis, and bioactivity of a male-aggrgation pheromone in assassin bug, Prithesancus plagipennis (Hemiptera: Reduviidae). J Chem Ecol 20:3281–3295

    Article  CAS  PubMed  Google Scholar 

  19. Jang SA, Cho JH, Park GM, Choo HY, Park CG (2011) Attraction of Gymnosoma rotundatum (Diptera: Tachinidae) to different amounts of Plautia stali (Hemiptera: Pentatomidae) aggregation pheromone and the effect of different pheromone dispensers. J Asia Pac Entomol 14:119–121

    Article  CAS  Google Scholar 

  20. Kugel M (1997) The time course of the electroretinogram of compound eyes in insects and its dependence on special recording conditions. J Exp Biol 71:1–6

    Google Scholar 

  21. Laumann RA, Borges M, Aldrich JR, Khrimian A, Blassioli-Moraes MC (2012) Attractant pheromone of the neotropical species Neomegalotomus parvus (Westwood) (Heteroptera: Alydidae). Psyche: J Entomol 2012:1–8

    Article  Google Scholar 

  22. Maruthadurai R, Gautam RD, Mahesh P (2011) Kairomonal effect of host body washing on the egg parasitoid Trichogramma brasiliensis (Ashmead) (Hymenoptera: Trichogrammatidae). J Biol Control 25:298–304

    Google Scholar 

  23. Njihia TN, Torto B, Murungi LK, Irungu J, Mwenda DM, Babin R (2017) Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae). Chemoecol 27:239–248

    Article  CAS  Google Scholar 

  24. R Development Core Team (2012) A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna

    Google Scholar 

  25. Sanou A, Traoré F, Ba MN, Dabiré-Binso CL, Pittendrigh BR, Sanon A (2019) Effects of volatiles from Clavigralla tomentosicollis Stål (Hemiptera: Coreidae) adults on the host location behavior of the egg parasitoid Gryon fulviventre (Crawford) (Hymenoptera: Scelionidae). Int J Insect Sci 11:1179543318825250

    Article  PubMed  PubMed Central  Google Scholar 

  26. Taylor TA (1975) Gryon gnidus, a scelionid egg-parasite of Acanthomia tomentosicollis (Hemiptera: Coreidae) in Nigeria. Entomophaga 20:129–134

    Article  Google Scholar 

  27. Wolde-Hawariat Y (2008) Identification of volatile compounds from ripe banana and attraction of sorghum chafer Pachnoda interrupta using y-tube olfactometer. In: Wolde-Hawariat Y (ed) Electrophysiological and behavioral responses of Sorghum chafer, Pachnoda interrupta (Coleoptera: Scarabaeiae) to host plant volatile compounds (pp 71–80). Dissertation, Addis Ababa University, Ethiopia

  28. Yasuda K (1998) Function of the male pheromone of the leaf footed plant bug, Leptoglossus australis (Fabricius) (Heteroptera: Coreidae) and its kairomonal effect. Japan Agric Res 32:161–165

    CAS  Google Scholar 

  29. Yusufoglu HS, Tabanca N, Bernier UR, Li AY, Salkini MA, Alqasoumi SI, Demirci B (2018) Mosquito and tick repellency of two anthemis essential oils from Saudi Arabia. Saudi Pharma J:1–5

  30. Zarbin PH, Fávaro CF, Vidal DM, Rodrigues MA (2012) Male-produced sex pheromone of the stink bug Edessa meditabunda. J Chem Ecol 38:825–835

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Mrs. Wanyama, Onesmus Kaye, Kirwa, Hillary Kipchirchir for the technical assistance and Mr. Xavier Cheseto for his contribution. We also acknowledge Dr. Elie A. Dannon from IITA-Bénin for the assistance and guidance during this work. We are grateful to the staff at the International Centre of Insect Physiology and Ecology (icipe), Duduville, Nairobi, for their support without which our research would not have been possible. Financial support for this research was provided by UK’s Department for International Development (DFID), Swedish International Development Cooperation Agency (Sida), the Swiss Agency for Development and Cooperation (SDC), Federal Democratic Republic of Ethiopia; and the Kenyan Government. Hilaire Kpongbe was supported by a German Academic Exchange Service (DAAD) In-Region Postgraduate Scholarship.

Author information

Affiliations

Authors

Contributions

HK, JVB, FK, MT, and BT conceived and designed the research. HK conducted experiments and analysed data. HK, JVB, FK, MT, and BT wrote the manuscript. EJT gave us support in the morphological identification of Gryon sp. All authors edited the manuscript and approved the final version.

Corresponding author

Correspondence to Baldwyn Torto.

Ethics declarations

Conflict of Interest

Authors declare no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Electronic supplementary material

ESM 1

(DOC 535 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kpongbe, H., Van Den Berg, J., Khamis, F. et al. Isopentyl Butanoate: Aggregation Pheromone of the Brown Spiny Bug, Clavigralla tomentosicollis (Hemiptera: Coreidae), and Kairomone for the Egg Parasitoid Gryon sp. (Hymenoptera: Scelionidae). J Chem Ecol 45, 570–578 (2019). https://doi.org/10.1007/s10886-019-01081-5

Download citation

Keywords

  • Aggregation pheromone
  • Biological control
  • Clavigralla tomentosicollis
  • Gryon sp.
  • Kairomone