Journal of Chemical Ecology

, Volume 45, Issue 4, pp 366–370 | Cite as

Identification of Aggregation-Sex Pheromone Components for a “Living Fossil”, the False Click Beetle, Palaeoxenus dohrni Horn (Coleoptera: Eucnemidae)

  • Jacqueline M. SerranoEmail author
  • J. Steven McElfresh
  • Yunfan Zou
  • Jocelyn G. Millar
Rapid Communication


Insect pheromones have rarely been exploited in surveys or studies of rare and endangered species, despite their potential as effective and highly selective attractants for target species. Here, we report the identification, synthesis, and field bioassays of a male-produced aggregation-sex pheromone blend of a rare false click beetle species endemic to southern California, Dohrn’s elegant eucnemid beetle, Palaeoxenus dohrni Horn (Coleoptera: Eucnemidae). This species is the only extant species in its genus and subfamily. Analyses of extracts of headspace volatiles collected from adult beetles revealed several male-specific compounds. Two of these compounds, identified as (E)-2-nonen-4-one and (R)-2-nonanol, elicited electroantennographic responses from antennae of beetles of both sexes. In field bioassays, a blend of the two compounds attracted both sexes, whereas the individual compounds were not attractive. The identification of an attractant pheromone should provide a useful tool for bioconservation and ecological studies of this iconic species.


Eucnemidae Aggregation-sex pheromone (E)-2-Nonen-4-one (R)-2-Nonanol False click beetle Saproxylic 



We thank Stacy Hishinuma for providing access to field sites, and Sean Halloran for assistance with field work. We also thank Ian Wright for assistance with specimen photography. This work was funded in part by Hatch Act grant #CA-R*-5181-H to JGM and Robert van den Bosch scholarship to JMS.

Supplementary material

10886_2019_1068_MOESM1_ESM.docx (688 kb)
ESM 1 (DOCX 687 kb)


  1. Cardé RT (2014) Defining attraction and aggregation pheromones: teleological versus functional perspectives. J Chem Ecol 40:519–520CrossRefGoogle Scholar
  2. Chang H, Muona J, Hanyong P, Chen W, Teräväinen M, Dong R, Qiang Y, Zingliao Z, Songhai J (2016) Chinese cretaceous larva exposes a southern Californian living fossil (Insecta, Coleoptera, Eucnemidae). Cladistics 32:211–214CrossRefGoogle Scholar
  3. Fenyes A (1903) Palaeoxenus (Cryptostoma) dohrnii horn. Entomol News 11:220Google Scholar
  4. Larsson MC (2016) Pheromones and other semiochemicals for monitoring rare and endangered species. J Chem Ecol 42:853–868CrossRefPubMedCentralGoogle Scholar
  5. Molander MA, Larsson MC (2018) Identification of the aggregation-sex pheromone of the cerambycid beetle Phymatodes pusillus ssp. pusillus and evidence of a synergistic effect from a heterospecific pheromone component. J Chem Ecol 44:987–998CrossRefPubMedCentralGoogle Scholar
  6. Molander MA, Helhesson J, Winde IB, Millar JG, Larsson MC (2019) The male-produced aggregation-sex pheromone of the cerambycid beetle Plagionotus detritus ssp. detritus. J Chem Ecol 45:28–36CrossRefGoogle Scholar
  7. Muona J (2000) A revision of the Nearctic Eucnemidae. Acta Zool Fenn 212:1–106Google Scholar
  8. Muona J (2002) Eucnemidae. In: Arnett RH, Thomas MC, Skelley PE, Frank JH (eds) American Beetles, vol 2. Polyphaga: Scarabaeoidea through Curculionoidea. CRC Press, Boca Raton, pp 152–157Google Scholar
  9. Muona J (2010) Eucnemidae Eschscholtz, 1829. In: Leschen RAB, Beutel RG, Lawrence JF (eds) Handbook of zoology. A natural history of the phyla of the animal kingdom. Vol. IV—Arthropoda: Insecta. Part 38. Coleoptera, beetles. Vol 2: systematics. Walter de Gruyter, Berlin, pp 574–659Google Scholar
  10. Newcombe RG (1998) Two-sided confidence intervals for the single proportion: comparison of seven methods. Stat Med 17:857–872CrossRefGoogle Scholar
  11. Ogle DH, Wheeler P, Dinno A (2018) FSA: fisheries stock analysis. R package version 0.8.22.
  12. Žunič Kosi A, Zou Y, Hoskovec M, Vrezec A, Stritih N, Millar JG (2017) Novel, male-produced aggregation pheromone of the cerambycid beetle Rosalia alpina, a priority species of European conservation concern. PLoS One 12:e0183279CrossRefPubMedCentralGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of EntomologyUniversity of CaliforniaRiversideUSA
  2. 2.Department of ChemistryUniversity of CaliforniaRiversideUSA

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