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Journal of Chemical Ecology

, Volume 44, Issue 3, pp 268–275 | Cite as

Interspecific Cross-Attraction between the South American Cerambycid Beetles Cotyclytus curvatus and Megacyllene acuta is Averted by Minor Pheromone Components

  • Weliton D. Silva
  • Jocelyn G. Millar
  • Lawrence M. Hanks
  • Camila M. Costa
  • Mariana O. G. Leite
  • Mateus Tonelli
  • José Maurício S. Bento
Article

Abstract

During field screening trials conducted in Brazil in 2015, adults of both sexes of the cerambycid beetles Cotyclytus curvatus (Germar) and Megacyllene acuta (Germar) (subfamily Cerambycinae, tribe Clytini) were significantly attracted to racemic 3-hydroxyhexan-2-one and racemic 2-methylbutan-1-ol, chemicals which previously have been identified as male-produced aggregation-sex pheromones of a number of cerambycid species endemic to other continents. Subsequent analyses of samples of beetle-produced volatiles revealed that males of C. curvatus sex-specifically produce only (R)-3-hydroxyhexan-2-one, whereas males of M. acuta produce the same compound along with lesser amounts of (2S,3S)-2,3-hexanediol and (S)-2-methylbutan-1-ol. Follow-up field trials showed that both sexes of both species were attracted to synthetic reconstructions of their respective pheromones, confirming that males produce aggregation-sex pheromones. The minor pheromone components of M. acuta, (S)-2-methylbutan-1-ol and (2S,3S)-2,3-hexanediol, synergized attraction of that species, but antagonized attraction of C. curvatus to (R)-3-hydroxyhexan-2-one. Beetles of other cerambycine species also were attracted in significant numbers, including Chrysoprasis linearis Bates, Cotyclytus dorsalis (Laporte & Gory), and Megacyllene falsa (Chevrolat). Our results provide further evidence that 3-hydroxyhexan-2-one is a major component of attractant pheromones of numerous cerambycine species world-wide. Our results also highlight our increasing understanding of the crucial role of minor pheromone components in imparting species specificity to cerambycid pheromone blends, as is known to occur in numerous species in other insect families.

Keywords

Coleoptera Longhorned beetle Reproductive isolation Mate location Semiochemical Pheromone chemistry 

Notes

Acknowledgments

We thank Antonio Santos-Silva (Museu de Zoologia da Universidade de São Paulo) for the identification of the cerambycid species. We gratefully acknowledge financial support from São Paulo Research Foundation - FAPESP (grant #2013/26936-2) to WDS and from USDA-APHIS (grant #15-8130-1422-CA) to JGM and LMH, and the Alphawood Foundation to LMH. This work was also supported by INCT-Semioquímicos na Agricultura (FAPESP and CNPq - grants #2014/50871-0 and #465511/2014-7, respectively). The field collections of the cerambycid beetles were conducted under the SISBIO permit n° #46395-2 from the Brazilian Ministry of the Environment.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Weliton D. Silva
    • 1
  • Jocelyn G. Millar
    • 2
  • Lawrence M. Hanks
    • 3
  • Camila M. Costa
    • 1
  • Mariana O. G. Leite
    • 1
  • Mateus Tonelli
    • 1
  • José Maurício S. Bento
    • 1
  1. 1.Department of Entomology and AcarologyUniversity of São PauloPiracicabaBrazil
  2. 2.Departments of Entomology and ChemistryUniversity of CaliforniaRiversideUSA
  3. 3.Department of EntomologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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