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Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella

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Abstract

Diterpene resin acids (DRAs) are important components of oleoresin and greatly contribute to the defense strategies of conifers against herbivorous insects. In the present study, we determined that DRAs function as insect juvenile hormone (JH) antagonists that interfere with the juvenile hormone-mediated binding of the JH receptor Methoprene-tolerant (Met) and steroid receptor coactivator (SRC). Using a yeast two-hybrid system transformed with Met and SRC from the Indian meal moth Plodia interpunctella, we tested the interfering activity of 3704 plant extracts against JH III-mediated Met-SRC binding. Plant extracts from conifers, especially members of the Pinaceae, exhibited strong interfering activity, and four active interfering DRAs (7α-dehydroabietic acid, 7-oxodehydroabietic acid, dehydroabietic acid, and sandaracopimaric acid) were isolated from roots of the Japanese pine Pinus densiflora. The four isolated DRAs, along with abietic acid, disrupted the juvenile hormone-mediated binding of P. interpunctella Met and SRC, although only 7-oxodehydroabietic acid disrupted larval development. These results demonstrate that DRAs may play a defensive role against herbivorous insects via insect endocrine-disrupting activity.

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Acknowledgements

This work was supported by a grant from the National Institute of Forest Science (Project No. FE0702-2016-02-2016 “Development of environment-friendly control agents against pine wilt disease based on BT”) and KRIBB Research Initiative Program (KGM4981612), awarded to H.O., and the National Research Foundation of Korea Grant (NRF-2008-2004725), awarded to D.P

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Author notes

  1. Hyun-Woo Oh, Chan-Seok Yun and Jun Hyoung Jeon contributed equally to this work.

Authors and Affiliations

  1. Core Facility Management Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Hyun-Woo Oh, Ji-Ae Kim & Sang Woon Shin

  2. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 580-185, Republic of Korea

    Chan-Seok Yun, Jun Hyoung Jeon & Doo-Sang Park

  3. Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chung-buk, 363-883, Republic of Korea

    Hyung Won Ryu & Sei-Ryang Oh

  4. Agency for Korea National Food Cluster, Iksan, 507-749, Republic of Korea

    Hyuk-Hwan Song

  5. Department of Integrative Biology, University of California, Berkley, CA, 94720, USA

    Yunhee Shin

  6. Division of Forest Insect Pests and Disease, Korea Forest Research Institute, Seoul, 130-712, Republic of Korea

    Chan Sik Jung

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  1. Hyun-Woo Oh
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  2. Chan-Seok Yun
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  3. Jun Hyoung Jeon
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  4. Ji-Ae Kim
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  5. Doo-Sang Park
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  9. Yunhee Shin
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  11. Sang Woon Shin
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Correspondence to Hyun-Woo Oh or Sang Woon Shin.

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Oh, HW., Yun, CS., Jeon, J.H. et al. Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella . J Chem Ecol 43, 703–711 (2017). https://doi.org/10.1007/s10886-017-0861-9

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  • DOI: https://doi.org/10.1007/s10886-017-0861-9

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