Effect of Brood Pheromone on Survival and Nutrient Intake of African Honey Bees (Apis mellifera scutellata) under Controlled Conditions
- 280 Downloads
The influence of pheromones on insect physiology and behavior has been thoroughly reported for numerous aspects, such as attraction, gland development, aggregation, mate and kin recognition. Brood pheromone (BP) is released by honey bee larvae to indicate their protein requirements to the colony. Although BP is known to modulate pollen and protein consumption, which in turn can affect physiological and morphological parameters, such as hypopharyngeal gland (HPG) development and ovarian activation, few studies have focused on the effect of BP on nutritional balance. In this study, we exposed newly emerged worker bees for 14 d and found that BP exposure increased protein intake during the first few days, with a peak in consumption at day four following exposure. BP exposure decreased survival of caged honey bees, but did not affect either the size of the HPG acini or ovarian activation stage. The uncoupling of the BP releaser effect, facilitated by working under controlled conditions, and the presence of larvae as stimulating cues are discussed.
KeywordsSemiochemical Primer and releaser effects Survival Nutrient intake Hypopharyngeal glands Savannah honey bee
FJD is supported by a postdoctoral fellowship from the University of Pretoria. The authors would like to thank Kendall Crous for beekeeping, Susana da Silva das Neves and Zoe van Vuuren for helping with dissections, and Alan Hall and the Laboratory for Microscopy and Microanalysis at the University of Pretoria for the HPG pictures.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Brand JM, Young JC, Silverstein RM (1979) Insect pheromones: a critical review of recent advances in their chemistry, biology, and application. In: Albersheim P, Brand JM, Darvill AG et al (eds) Fortschritte der Chemie organischer Naturstoffe/progress in the chemistry of organic natural products. Springer Vienna, Vienna, pp 1–190CrossRefGoogle Scholar
- Mohammedi A, Crauser D, Paris A, Le Conte Y (1996) Effect of a brood pheromone on honeybee hypopharyngeal glands. C R Acad Sci III Sci Vie 319:769–772Google Scholar
- Pankiw T, Page RE (2003) Effect of pheromones, hormones, and handling on sucrose response thresholds of honey bees (Apis mellifera L.). J Comp Physiol A Neuroethol Sens Neural Behav Physiol 189(9):675–684Google Scholar
- Pankiw T, Lafontaine JP, Avelino N (2010) Stabilized brood pheromone for manipulating the behavior and physiology of honey bees United States Patent No: 7727517Google Scholar
- Winston ML (1991) The biology of the honey bee. Harvard University Press, CambridgeGoogle Scholar