Identification and Field Evaluation of Fermentation Volatiles from Wine and Vinegar that Mediate Attraction of Spotted Wing Drosophila, Drosophila suzukii
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Previous studies suggest that olfactory cues from damaged and fermented fruits play important roles in resource recognition of polyphagous spotted wing Drosophila flies (SWD), Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). They are attracted to fermented sweet materials, such as decomposing fruits but also wines and vinegars, and to ubiquitous fermentation volatiles, such as acetic acid and ethanol. Gas chromatography coupled with electroantennographic detection (GC-EAD), gas chromatography-mass spectrometry (GC-MS), two-choice laboratory bioassays, and field trapping experiments were used to identify volatile compounds from wine and vinegar that are involved in SWD attraction. In addition to acetic acid and ethanol, consistent EAD responses were obtained for 13 volatile wine compounds and seven volatile vinegar compounds, with all of the vinegar EAD-active compounds also present in wine. In a field trapping experiment, the 9-component vinegar blend and 15-component wine blend were similarly attractive when compared to an acetic acid plus ethanol mixture, but were not as attractive as the wine plus vinegar mixture. In two-choice laboratory bioassays, 7 EAD-active compounds (ethyl acetate, ethyl butyrate, ethyl lactate, 1-hexanol, isoamyl acetate, 2-methylbutyl acetate, and ethyl sorbate), when added singly to the mixture at the same concentrations tested in the field, decreased the attraction of SWD to the mixture of acetic acid and ethanol. The blends composed of the remaining EAD-active chemicals, an 8-component wine blend [acetic acid + ethanol + acetoin + grape butyrate + methionol + isoamyl lactate + 2-phenylethanol + diethyl succinate] and a 5-component vinegar blend [acetic acid + ethanol + acetoin + grape butyrate + 2-phenylethanol] were more attractive than the acetic acid plus ethanol mixture, and as attractive as the wine plus vinegar mixture in both laboratory assays and the field trapping experiment. These results indicate that these volatiles in wine and vinegar are crucial for SWD attraction to fermented materials on which they feed as adults.
KeywordsDrosophila suzukii Spotted wing Drosophila Feeding attractant Trap Lure GC-EAD
- Becher, P. G., Flick, G., Rozpędowska, E., Schmidt, A., Hagman, A., Lebreton, S., Larsson, M. C., Hansson, B. S., Piškur, J., Witzgall, P., and Bengtsson, M. 2012. Yeast, not fruit volatiles mediate Drosophila melanogaster attraction, oviposition and development. Funct. Ecol. 26:822–828.CrossRefGoogle Scholar
- Cha, D. H., Powell, T. H. Q., Feder, J. L., and Linn Jr., C. E. 2011b. Identification of host fruit volatiles from three mayhaw species (Crataegus Series Aestivales) attractive to mayhaw-origin Rhagoletis pomonella flies in the Southern United States. J. Chem. Ecol. 37:961–973.PubMedCrossRefGoogle Scholar
- Cha, D. H., Powell, T. H. Q., Feder, J. L., and Linn Jr., C. E. 2011c. Identification of fruit volatiles from green hawthorn (Crataegus viridis) and blueberry hawthorn (Crataegus brachyacantha) host plants attractive to different phenotypes of Rhagoletis pomonella flies in the Southern United States. J. Chem. Ecol. 37:974–983.PubMedCrossRefGoogle Scholar
- Cha, D. H., Yee, W. L., Goughnour, R. B., Sim, S. B., Powell, T. H. Q., Feder, J. L., and Linn Jr., C. E. 2012b. Identification of host fruit volatiles from domestic apple (Malus domestica), native black hawthorn (Crataegus douglasii) and introduced ornamental hawthorn (C. monogyna) attractive to Rhagoletis pomonella flies from the Western United States. J. Chem. Ecol. 38:319–329.PubMedCrossRefGoogle Scholar
- Cini, A., Ioriatti, C., and Anfora, G. 2012. A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management. Bull. Insect. 65:149–160.Google Scholar
- Cossé, A. A. and Baker, T. C. 1996. House flies and pig manure volatiles: wind tunnel behavioral studies and electrophysiological evaluations. J. Agric. Entomol. 13:301–317.Google Scholar
- Cossé, A. A., Todd, J. L., Millar, J. G., Martínez, L. A., and Baker, T. C. 1995. Electroantennographic and coupled gas chromatographic-electroantennographic responses of the mediterranean fruit fly, Ceratitis capitata, to male-produced volatiles and mango odor. J. Chem. Ecol. 21:1823–1836.CrossRefGoogle Scholar
- Davis, T. S., Boundy-Mills, K., and Landolt, P. J. 2012. Volatile emissions from an epiphytic fungus are semiochemicals for eusocial wasps. Microb. Ecol. doi:10.1007/s00248-012-0074-2.
- Delfinado, M. D. and Hardy, D. E. 1977. A Catalog of the Diptera of the Oriental Region. Volume III. Suborder Cyclorrapha. The University Press of Hawaii, Honolulu.Google Scholar
- Dethier, V. G. 1947. Chemical Insect Attractants and Repellents. Maple Press Co., York, OA.Google Scholar
- Hayashibe, M., Katoda, S., Owada, H., Yoshida, H., Katayose, A., and Terashim, T. 1970. Methionine metabolism in yeast.1. methionol formation. J. Ferm. Tech. 48:22–28.Google Scholar
- Hilker, M. and McNeil, J. 2007. Chemical and behavioral ecology in insect parasitoids: how to behave optimally in a complex odourous environment, pp. 92–112, in E. Wajnberg, C. Bernstein, and J. van Alphen (eds.), Behavioral Ecology of Insect Parasitoids. Blackwell Publishing, Malden, MA.Google Scholar
- Kaneshiro, K. Y. 1983. Drosophila (Sophophora) suzukii (Matsumura). Proc. Hawaiian Entomol. Soc. 24:179.Google Scholar
- Kanzawa, T. 1934. Research into the fruit fly Drosophila suzukii Matsura. Yamanashi Prefecture Agricultural Experiment Station Report, October 1934, 48 p.Google Scholar
- Landolt, P. J., Adams, T., Davis, T., and Rogg, H. 2012b. Spotted wing Drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), trapped with combinations of wines and vinegars. Flo. Entomol. 95:326–332.Google Scholar
- McDonald, J. H. 2009. Handbook of Biological Statistics, 2nd ed. Sparky House Publishing, Baltimore, MD, USA.Google Scholar
- Reed, M. R. 1938. The olfactory reactions of Drosophila melanogaster Meigen to the products of fermenting banana. Physiol. Zool. 11:317–325.Google Scholar
- Rodan, A. R. and Rothenfluh, A. 2010. The genetics of behavioral alcohol responses in Drosophila, pp. 25–51, in M. T. Reilly and D. M. Lovinger (eds.), Functional Plasticity and Genetic Variation: Insights into the Neurobiology and Alcoholism. Elsevier Academic Press, San Diego, CA.CrossRefGoogle Scholar
- Roelofs, W. L. and Cardé, R. T. 1974. Oriental fruit moth and lesser appleworm attractant mixtures refined. Environ. Entomol. 3:586–588.Google Scholar
- SAS Institute. 2009. The mixed model procedure, version 9.2. www.sas.com.
- Schoonhoven, L. M., Jermy, T., and van Loon, J. J. A. 1998. Insect-Plant Biology. Chapman & Hall, London, UK.Google Scholar
- Steck, G. J., Dixon, W. and Dean, D. 2009. Spotted wing Drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), a fruit pest new to North America. Pest Alerts. www.fl.dpi.com/enpp/ento/drosophila_suzukii.html.
- Wagner, K. and Rapp, A. 1999. Influence of yeasts on the formation of 2-phenylethanol during the alcoholic fermentation. Dtsch Lebensm Rundsch 95:304–309.Google Scholar
- Walsh, D. B., Bolda, M. P., Goodhow, R. E., Dreves, A. J., Lee, J., Bruck, D. V., Walton, M., O’NEAL, S. D., and Zalom, F. G. 2011. Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential. J. Integ. Pest Manag. 106:289–295.Google Scholar
- Witzgall, P., Proffit, M., Rozpedowska, E., Becher, P. G., Andreadis, S., Coracini, M., Lindblom, T. U., Ream, L. J., Hagman, A., Bengtsson, M., Kurtzman, C. P., Piskur, J., and Knight, A. 2012. “This is not an apple”-yeast mutualism in codling moth. J. Chem. Ecol. 38:949–957.PubMedCrossRefGoogle Scholar
- Zar, J. H. 1984. Biostatistical Analysis. Prentice-Hall, New Jersey.Google Scholar