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Journal of Chemical Ecology

, 37:1117 | Cite as

Genetic Relatedness and Chemical Profiles in an Unusually Peaceful Eusocial Bee

  • Sara Diana LeonhardtEmail author
  • Sven Form
  • Nico Blüthgen
  • Thomas Schmitt
  • Heike Feldhaar
Article

Abstract

Colonies of the stingless bee Tetragonilla collina frequently occur in unusually high densities and in direct neighborhood (nest aggregations), in rainforests of Southeast Asia. To investigate whether close relatedness and/or similar chemical profiles facilitate the co-occurrence of multiple T. collina colonies, we investigated aggressive behavior, genetic relatedness and cuticular hydrocarbon (CHC) profiles within and between colonies and nest aggregations. Although 17 out of 19 colonies within aggregations were largely unrelated, intraspecific aggression between different colonies was basically absent both within and among aggregations. This lack of aggression should favor social parasitism and hence the occurrence of unrelated individuals within a colony. However, low within-colony relatedness was found in only five out of 19 colonies where it may be explained by queen turnover or the occurrence of foreign workers. CHC profiles of colonies within and among aggregations were statistically different. However, many workers could chemically not be assigned to their maternal colony, indicating considerable overlap among colonies in odor profiles of workers. Moreover, odor profiles tended to be more similar within than among aggregations, although most colonies were unrelated. Thus, CHC profiles were a poor indicator of relatedness in T. collina. The lack of correlation between relatedness and chemical similarity in T. collina may be explained by the incorporation of resin-derived terpenes in their CHC profiles. The composition of these terpenes was highly similar among colonies, particularly within aggregations, hence potentially decreasing chemical distinctiveness and increasing behavioral tolerance.

Key Words

Aggregated nesting Aggressive behavior Nestmate recognition Gas-chromatography mass-spectrometry Microsatellite analysis Stingless bees 

Notes

Acknowledgements

We thank the Economic Planning Unit (EPU) for giving us permission to perform research in Malaysia, and the Royal Society for their help with planning and conducting field work in Borneo. This project was further kindly supported by Dr. Chey Vun Khen, Dr. Arthur Chung and Dr. Robert Ong (Forestry Research Centre, Sandakan). Ben Oldroyd and Emilie Cameron provided additional data for comparison and validation of our results and Linda-Maria Jung helped with data collection. The comments of two anonymous reviewers greatly improved the manuscript. SDL was supported by a grant of the German Excellence Initiative to the Graduate School of Life Science, University of Würzburg, NB, HF and TS by the Sonderforschungsbereich SFB 554 (Mechanisms and Evolution of Arthropod Behaviour) of the Deutsche Forschungsgemeinschaft (DFG).

Supplementary material

10886_2011_16_MOESM1_ESM.ppt (136 kb)
Figure S1 Similarity in chemical profiles of surface compounds from 14 Tetragonilla colonies of five nest aggregations in Borneo (ordination figure based on Bray-Curtis dissimilarities, stress value = 10.73 (note that stress values below 15 are generally accepted as reliable, see Backhaus et al 2003: Multivariate Analysemethoden. Eine Anwendungsorientierte Einführung. Springer, Berlin)). Different symbols represent different colonies (each symbol represents one individual bee): two colonies were collected from an aggregation in DVC (black and white upright ellipses) and four colonies from an aggregation found close to the coast in KSR (black and white ellipses, black and white triangles upside down). The remaining colonies stem from one aggregation in the interior forest of KSR (black diamonds, white circles, black and grey triangles), and two aggregations at the RDC (white diamond and black square as well as grey squares and black circles). Grey symbols mark two Tetragonilla spec. colonies (grey triangle and square) that are morphologically highly similar to T. collina, but strongly differ in their chemical profiles. These colonies most likely represent another Tetragonilla species and were thus excluded from further analyses. (PPT 136 kb)
10886_2011_16_MOESM2_ESM.ppt (263 kb)
Figure S2 Chromatograms of body surfaces from individual bees. Hexane extracts of two T. collina bees (a & b) and one Tetragonilla spec. (c) from nest aggregations in the RDC. Chemical differences between the two species are primarily based on esters and germacrene-D. (PPT 263 kb)
10886_2011_16_MOESM3_ESM.doc (71 kb)
Table S1 (DOC 71 kb)
10886_2011_16_MOESM4_ESM.doc (180 kb)
Table S2 (DOC 179 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Sara Diana Leonhardt
    • 1
    Email author
  • Sven Form
    • 3
  • Nico Blüthgen
    • 2
  • Thomas Schmitt
    • 4
  • Heike Feldhaar
    • 5
  1. 1.Department of EcologyUniversity of LüneburgLüneburgGermany
  2. 2.Department of BiologyUniversity of DarmstadtDarmstadtGermany
  3. 3.Department of PsychologyUniversity of KielKielGermany
  4. 4.Department of Evolutionary Biology and Animal Ecology, Faculty of BiologyUniversity of FreiburgFreiburgGermany
  5. 5.Department of Animal EcologyUniversity of BayreuthBayreuthGermany

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