Skip to main content
Log in

The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females

  • Published:
Journal of Chemical Ecology Aims and scope Submit manuscript

Abstract

Females of the pine sawfly Neodiprion sertifer (Hymenoptera Diprionidae) usually avoid Pinus pinea trees as host plants. In contrast, this sawfly species is highly attracted by P. sylvestris and P. nigra trees. Here, we investigated which pine volatiles might mediate this behavior by in situ sampling experiments and olfactometer laboratory tests. Volatiles emitted from P. pinea, P. sylvestris, and P. nigra foliage were sampled by solid-phase microextraction (SPME). Analysis of these volatiles by coupled gaschromatography/mass spectrometry revealed that the relative amounts of the compounds emitted by the three species were significantly different. A discriminant analysis showed that the amounts of limonene and myrcene significantly contributed to the species-specific volatile patterns. Pinus pinea emitted higher relative amounts of limonene than the other pine species. Pinus sylvestris emitted the highest relative amounts of myrcene. When testing the response of N. sertifer females to these pine terpenoids in an olfactometer bioassay, a low amount of limonene was attractive, while a repellent effect was evident when higher amounts were used. The sawfly females showed no significant olfactory response to myrcene. These data suggest that low relative amounts of limonene have a significant function in attracting N. sertifer females, while high amounts might contribute to avoidance of a tree.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Adams, R. P. 2007. Identification of essential oil components by gas chromatography/mass spectrometry. 4th edn. Allured Publishing Corporation, Carol Stream. p. 804.

    Google Scholar 

  • Annila, E., and Hiltunen, R. 1977. Damage by Pissodes validirostris (Coleoptera, Curculionidae) studied in relation to the monoterpene composition in Scots pine and lodgepole pine. Ann. Entomol. Fenn. 43:87–92.

    CAS  Google Scholar 

  • Asaro, C., Sullivan, B. T., Dalusky, M. J., and Berisford, C. W. 2004. Volatiles associated with preferred and nonpreferred hosts of the nantucket pine tip moth, Rhyacionia frustrana. J. Chem. Ecol. 30:977–990.

    Article  CAS  PubMed  Google Scholar 

  • Austarå, Ø., Orlund, A., and Svendsrud, A. 1987. Growth loss and economic consequences following two years defoliation of Pinus sylvestris by the pine sawfly Neodiprion sertifer in West-Norway. Scand. J. For. Res. 2:111–119.

    Article  Google Scholar 

  • Baldassari, N., Martini, A., and Baronio, P. 2003. A technique for continuous rearing of Neodiprion sertifer (Geoffroy) in the laboratory. J. Appl. Entomol. 127:103–106.

    Article  Google Scholar 

  • Baronio, P., Martini, A., and Baldassari, N. 1997. Neodiprion sertifer pericoloso nemico del pino silvestre. Inftore Fitopatol. 47:12–20.

    Google Scholar 

  • Bocchini, P., Dello Monaco, D., Pozzi, R., Pinelli, F., and Galletti, G. C. 2009. Solid-phase microextraction coupled to gas chromatography with flame ionization detection for monitoring of organic solvents in working areas. Microchim. Acta 165:271–278.

    Article  CAS  Google Scholar 

  • Breny, R., and Detroux, L. 1950. Considèrations sur la biologie et la nuisance de Neodiprion sertifer Geoffr. et rapport sur les traitements effectuès en 1949 dans les pineraies de la règion de Spa. Parasitica 6:123–138.

    CAS  Google Scholar 

  • Chai, M., and Pawliszyn, J. 1995. Analysis of environmental air samples by solid-phase microextraction and gas chromatography/ion trap mass spectrometry. Environ. Sci. Technol., 29:693–701.

    Article  CAS  Google Scholar 

  • Chai, M., Arthur, C. L., Pawliszyn, J., Belardi, R. P., and Pratt, K. 1993. Determination of volatile chlorinated hydrocarbons in air and water with solid-phase microextraction. Analyst 118:1501–1505.

    Article  CAS  Google Scholar 

  • Koschier, E. H., De Kogel, and W. J., Visser, J. H. 2000. Assessing the attractiveness of volatile plant compound to western flower thrips Frankliniella occidentalis. J. Chem. Ecol. 26:2643–2655.

    Article  CAS  Google Scholar 

  • Kozlowski, T. T., and Winget, C. H. 1964. The role of reserves in leaves, branches, stems and roots on growth of red pine. Am. J. Bot. 5:522–529.

    Article  Google Scholar 

  • Lyons, L. A. 1964. The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae). A review with emphasis on studies in Ontario. Proc. Ent. Soc. Ont. 94:5–37.

    Google Scholar 

  • Ouyang, G., and Pawliszyn, J. 2006. Recent developments in SPME for on-site analysis and monitoring. Trends Anal. Chem. 25:692–703.

    Article  CAS  Google Scholar 

  • Pschorn-Walcher, H. 1965. The ecology of Neodiprion sertifer (Geoffr.) (Hym.: Diprionidae) and a review of its parasite complex in Europe. Tech. Bull. Commonw. Inst. Biol. Control 5:33–97.

    Google Scholar 

  • Pschorn-Walcher, H. 1970. Studies on the biology and ecology of the alpine form of Neodiprion sertifer (Geoffr.) (Hym: Diprionidae) in the Swiss Alps. Z. Angew. Entomol. 66:64–83.

    Google Scholar 

  • Rivers, C. F. 1962. The use of polyhedral virus disease in the control of pine sawfly, Neodiprion sertifer (Geoffr.) in north west Scotland. Entomophaga, mémoire hors série numéro 2:477-478.

    Google Scholar 

  • Sabelis, M. W., and Van De Baan, H. E. 1983. Location of distant spider mite colonies by phytoseid predators: demonstration of specific kairomones emitted by Tetranychus urticae and Panonychus ulmi. Entomol. Exp. Appl. 33:303–314.

    Google Scholar 

  • STATSOFT INC 1995. Statistica. 2nd edn, Tulsa, (OK), USA.

  • Tiberi, R, Niccoli, A., Curini, M., Epifanio, F., Marcotullio, M. C., and Rosati, O. 1999. The role of the monoterpene composition in Pinus spp. needles, in host selection by the pine processionary caterpillar, Thaumetopoea pityocampa. Phytoparasitica 27:263–272.

    Article  CAS  Google Scholar 

  • Xiong, G., Koziel, J., and Pawliszyn, J. 2004. Air sampling of aromatic hydrocarbons in the presence of ozone by solid-phase microextraction. J. Chromatogr. A 1025:57–62.

    Article  CAS  PubMed  Google Scholar 

  • Yassaa, N., and Williams, J. 2007. Enantiomeric monoterpene emission from natural and damaged Scots pine in a boreal coniferous forest measured using solid-phase microextraction and gas chromatography/mass spectrometry. J. Chromatogr. A 1141:138–144.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Antonio Martini.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martini, A., Botti, F., Galletti, G. et al. The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females. J Chem Ecol 36, 1114–1121 (2010). https://doi.org/10.1007/s10886-010-9851-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10886-010-9851-x

Key Words

Navigation