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Interactive Influence of Leaf Age, Light Intensity, and Girdling on Green Ash Foliar Chemistry and Emerald Ash Borer Development

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Abstract

Biotic and abiotic environmental factors affect plant nutritional quality and defensive compounds that confer plant resistance to herbivory. Influence of leaf age, light availability, and girdling on foliar nutrition and defense of green ash (Fraxinus pennsylvanica Marsh) was examined in this study. Longevity of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), adults reared on green ash foliage subjected to these factors was assayed. Mature leaves generally were more nutritious with greater amino acids and a greater ratio of protein to non-structural carbohydrate (P:C) than young leaves, in particular when trees were grown in shade. On the other hand, mature leaves had lower amounts of trypsin and chymotrypsin inhibitors, and total phenolics compared to young leaves. Lower defense of mature leaves alone, or along with higher nutritional quality may lead to increased survival and longevity of emerald ash borer feeding on mature leaves. Sunlight reduced amino acids and P:C ratio, irrespective of leaf age and girdling, and elevated total protein of young foliage, but not protein of mature leaves. Sunlight also dramatically increased all investigated defensive compounds of young, but not mature leaves. Girdling reduced green ash foliar nutrition, especially, of young leaves grown in shade and of mature leaves grown in sun. However emerald ash borer performance did not differ when fed leaves from trees grown in sun or shade, or from girdled or control trees. One explanation is that emerald ash borer reared on lower nutritional quality food may compensate for nutrient deficiency by increasing its consumption rate. The strong interactions among leaf age, light intensity, and girdling on nutrition and defense highlight the need for caution when interpreting data without considering possible interactions.

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Acknowledgments

Tina Ciaramitaro and Deborah Miller maintained the EAB colony and trees in the greenhouse. Drs. Dan Herms (Department of Entomology, the Ohio State University, OH), James Miller (Department of Entomology, the Michigan State University, MI), and two anonymous reviewers provided critical reviews of an earlier draft of the manuscript. Statistical Consulting Center (College of Agriculture and Natural Resources in Michigan State University) assisted with part of the statistical analyses. The authors thank all of these individuals and organizations. The research was supported by the USDA Forest Service, NA FHP.

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  1. Department of Entomology, Michigan State University, East Lansing, MI, 48824, USA

    Yigen Chen

  2. USDA Forest Service, Northern Research Station, East Lansing, MI, 48823, USA

    Therese M. Poland

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  1. Yigen Chen
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Correspondence to Yigen Chen.

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Chen, Y., Poland, T.M. Interactive Influence of Leaf Age, Light Intensity, and Girdling on Green Ash Foliar Chemistry and Emerald Ash Borer Development. J Chem Ecol 35, 806–815 (2009). https://doi.org/10.1007/s10886-009-9661-1

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