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Amphibian Chemical Defense: Antifungal Metabolites of the Microsymbiont Janthinobacterium lividum on the Salamander Plethodon cinereus

Abstract

Disease has spurred declines in global amphibian populations. In particular, the fungal pathogen Batrachochytrium dendrobatidis has decimated amphibian diversity in some areas unaffected by habitat loss. However, there is little evidence to explain how some amphibian species persist despite infection or even clear the pathogen beyond detection. One hypothesis is that certain bacterial symbionts on the skin of amphibians inhibit the growth of the pathogen. An antifungal strain of Janthinobacterium lividum, isolated from the skin of the red-backed salamander Plethodon cinereus, produces antifungal metabolites at concentrations lethal to B. dendrobatidis. Antifungal metabolites were identified by using reversed phase high performance liquid chromatography, high resolution mass spectrometry, nuclear magnetic resonance, and UV-Vis spectroscopy and tested for efficacy of inhibiting the pathogen. Two metabolites, indole-3-carboxaldehyde and violacein, inhibited the pathogen’s growth at relatively low concentrations (68.9 and 1.82 μM, respectively). Analysis of fresh salamander skin confirmed the presence of J. lividum and its metabolites on the skin of host salamanders in concentrations high enough to hinder or kill the pathogen (51 and 207 μM, respectively). These results support the hypothesis that cutaneous, mutualistic bacteria play a role in amphibian resistance to fungal disease. Exploitation of this biological process may provide long-term resistance to B. dendrobatidis for vulnerable amphibians and serve as a model for managing future emerging diseases in wildlife populations.

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Acknowledgements

This work was supported by a Research Corporation Cottrell College Science Award (KPCM), the Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust (KPCM), a National Science Foundation Research in Undergraduate Institutions grant 0640373 (RNH), and James Madison University. HR-MS analyses were conducted by the Mass Spectrometry Facility at Harvard University (Cambridge, MA, USA). The authors would like to thank Dr. Nancy Staub for mucus depth discussions and Dr. Grace Wyngaard for a critical reading of the manuscript.

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Correspondence to Kevin P. C. Minbiole.

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Supplementary Figure 1

RP-HPLC UV-Vis spectra of Janthinobacterium lividum extract, indole-3-carboxaldehyde standard, and violacein standard. UV-Vis spectra of (a) indole-3-carboxaldehyde and (b) violacein. Dashed lines refer to standards and solid lines refer to extracts from J. lividum. For both standards and extracts, the RP-HPLC retention time of indole-3-carboxaldehyde was approximately 9.06 min and violacein was approximately 11.15 min. (DOC 143 KB)

Supplementary Figure 2

A composite* of 16S rRNA gene DGGE profiles of skin bacteria from the wild caught salamanders, Plethodon cinereus, and comparison with migration of 16S rRNA gene fragments of Janthinobacterium lividum. The J. lividum standard (right lane) run along with the wild-caught salamanders and the resulting bands were matched (indicated with an arrow). *A composite of two separate gels with the same standard for comparison. Lane one is superimposed from an analogous DGGE. (DOC 19.5 KB)

Supplementary Table 1

1H NMR (600 MHz) and HR-MS analysis of antifungal compounds isolated from a broth culture of Janthinobacterium lividum. (DOC 26.5 KB)

Supplementary Table 2

Skin mucus depth determinations of Plethodon cinereus. a (DOC 61.5 KB)

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Brucker, R.M., Harris, R.N., Schwantes, C.R. et al. Amphibian Chemical Defense: Antifungal Metabolites of the Microsymbiont Janthinobacterium lividum on the Salamander Plethodon cinereus . J Chem Ecol 34, 1422–1429 (2008). https://doi.org/10.1007/s10886-008-9555-7

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  • DOI: https://doi.org/10.1007/s10886-008-9555-7

Keywords

  • Janthinobacterium lividum
  • Plethodon cinereus
  • Batrachochytrium dendrobatidis
  • Beneficial bacteria
  • Violacein
  • Indole-3-carboxaldehyde