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Identification and Molecular Cloning of Putative Odorant-Binding Proteins and Chemosensory Protein from the Bethylid Wasp, Scleroderma guani Xiao et Wu

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Abstract

Two putative odorant-binding proteins (OBPs) and one putative chemosensory protein (CSP) from females of the ant-like bethylid wasp, Scleroderma guani Xiao et Wu (Hymenoptera: Bethylidae), were identified and cloned. The putative OBPs and CSP were identified by nondenaturing polyacrylamide gel electrophoresis (native-PAGE). 3′ rapid amplification of cDNA ends (3′RACE) was performed to obtain the sequences of the mature proteins by using degenerate primers designed from N-terminal sequences. Gene-specific primers for 5′ rapid amplification of cDNA ends (5′RACE) were designed according to 3′RACE results and used in polymerase chain reaction (PCR) to obtain full-length sequences. The proteins (Sgua-OBP1, Sgua-OBP2, and Sgua-CSP1) encode 133, 142, and 129 amino acid-deduced sequences, respectively. Prediction of signal peptide sequences matches the N-terminal amino acid sequence of the isolated proteins. Database searches suggest that the Sgua-OBP1 and Sgua-OBP2 are homologs of OBPs from other insects, and Sgua-CSP1 shares a high level of identity with previously described CSPs.

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Acknowledgments

This work was supported by the China National Basic Research Program (973 Program) of Invasion Biology and Control Strategies for Alien Species in Agriculture and Forestry (2002CB111407). We also thank Dr. Patricia Nagnan-Le Meillour (INRA, Versailles, France) for her advice with this work.

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  1. Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100094, China

    Daguang Lu, Xiangrui Li, Xiaoxia Liu & Qingwen Zhang

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  1. Daguang Lu
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  2. Xiangrui Li
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  3. Xiaoxia Liu
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  4. Qingwen Zhang
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Correspondence to Qingwen Zhang.

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Daguang Lu and Xiangrui Li contributed equally to this work

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Lu, D., Li, X., Liu, X. et al. Identification and Molecular Cloning of Putative Odorant-Binding Proteins and Chemosensory Protein from the Bethylid Wasp, Scleroderma guani Xiao et Wu. J Chem Ecol 33, 1359–1375 (2007). https://doi.org/10.1007/s10886-007-9310-5

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