Chemical Identification of MHC-influenced Volatile Compounds in Mouse Urine. I: Quantitative Proportions of Major Chemosignals
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The genes of the major histocompatibility complex (MHC) are highly polymorphic loci that encode cell surface proteins, class I and II molecules. They present peptide antigens to T cells and thereby control immunological self/nonself recognition. Increasing evidence indicates that MHC genes also influence odor and mating preferences; however, it is unclear how. Here we report the results of chemical analyses of male mouse urinary odors collected from a variety of mouse strains, including MHC-congenics, recombinants, mutants, and transgenics (i.e., β2 microglobulin “knockouts,” which lack class I expression, and transporters associated with antigen processing (TAP) knock-outs). After the identification of volatile odor components by gas chromatography/mass spectrometry, the odor profiles of urine samples were analyzed quantitatively by using stir bar sorptive extraction and gas chromatography/atomic emission detection. Results showed that MHC genes influenced the amounts of testosterone-mediated pheromones, sulfur-containing compounds, and several carbonyl metabolites. This is the first report to quantitatively link known mouse pheromones to classical, antigen-binding MHC loci. Surprisingly, these compounds were not influenced by TAP genes, even though these loci are MHC-linked and play a role in peptide presentation. Whereas identification of MHC-determined odorants does not reveal their metabolic origin, some constituents were also present in blood serum, and their levels were not altered by antibiotics.
KeywordsIndividual odor Volatile pheromones Gas chromatography/mass spectrometry Gas chromatography/atomic emission detection MHC genes Mouse urine
- Novotny, M., Jorgenson, J. W., Carmack, M., Wilson, S. R., Boyse, E. A., Yamazaki, K., Wilson, M., Beamer, W., and Whitten, W. K. 1980. Chemical studies of the primer mouse pheromones, pp. 377–390, in D. Müller-Schwarze and R. M. Silverstein (eds.). Chemical Signals in Vertebrates and Aquatic Invertebrates. Plenum Press, New York.Google Scholar
- Novotny, M. V., Ma, W., Zidek, L., and Daev, E. 1999a. Recent biochemical insights into puberty acceleration, estrus induction, and puberty delay in the house mouse, pp. 99–116, in R. E. Johnston, D. Müller-Schwarze, and P. Sorenson (eds.). Advances in Chemical Signals in Vertebrates. Kluwer Academic/Plenum Publishers, New York.Google Scholar
- Schellinck, H. M., Brown, R. E., and Slotnick, B. M. 1991. Training rats to discriminate between the odors of individual conspecifics. Anim. Learn. Behav. 19:223–233.Google Scholar
- Willse, A., Belcher, A. M., Preti, G., Wahl, J. H., Thresher, M., Yang, P., Yamazaki, K., and Beauchamp, G. K. 2005. Identification of major histocompatibility complex-regulated body odorants by statistical analysis of a comparative gas chromatography/mass spectrometry experiment. Anal. Chem. 77:2348–2361.PubMedCrossRefGoogle Scholar