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Synthesis, Characterization and Employed Doxycycline Capped Gold Nanoparticles on TRP Channel Expressions in SKBR3 Breast Cancer Cells and Antimicrobial Activity

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Abstract

Doxycycline capped gold nanoparticles (doxy-Au(0) NPs) were prepared in an aqueous medium. Particle size and shape were determined by Transmission electron microscopy which showed the monodispersed morphology. The Fourier transform infrared spectra were represented the interaction of doxycycline with surface of doxy-Au(0) NPs. X-ray powder diffraction study gave crystalline nature of the doxy-Au(0) NPs. These doxy-Au(0) NPs have an impact on the expression levels of TRP channel genes in the breast cancer line (SKBR3) and the breast epithelial cell line (CRL-4010). Additionally, the antimicrobial activities have been evaluated to be used for multiple applications. Our results showed that the expression levels of TRP genes can be changed following the treatment of AuNPs (50 and 100 µg/mL). Furthermore, the antimicrobial activies have achieved some significant results. Finally, it has been concluded that doxy-Au(0) NPs are novel and can be used as alternative nanomedicine and extendable for control of other reducible contaminants as well.

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Funding

The authors would like to acknowledge and thanks Gaziantep University for logistic support.

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Authors and Affiliations

  1. Department of Biology, Division of Molecular Biology and Genetics, Gaziantep Univeristy, Gaziantep, 27000, Turkey

    Muhammad Safdar & Mehmet Ozaslan

  2. Cholistan University of Veterinary & Animal Sciences, Bahawalpur, 63100, Pakistan

    Muhammad Safdar & Yasmeen Junejo

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  1. Muhammad Safdar
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  2. Mehmet Ozaslan
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  3. Yasmeen Junejo
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All the authors have equally contributed in this manuscript.

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Correspondence to Mehmet Ozaslan or Yasmeen Junejo.

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Safdar, M., Ozaslan, M. & Junejo, Y. Synthesis, Characterization and Employed Doxycycline Capped Gold Nanoparticles on TRP Channel Expressions in SKBR3 Breast Cancer Cells and Antimicrobial Activity. J Clust Sci 33, 2635–2642 (2022). https://doi.org/10.1007/s10876-021-02181-7

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  • DOI: https://doi.org/10.1007/s10876-021-02181-7

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