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Comparison Between β-Cyclodextrin-Amygdalin Nanoparticle and Amygdalin Effects on Migration and Apoptosis of MCF-7 Breast Cancer Cell Line

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Abstract

Amygdalin is an herbal cyanoglycoside that has an anti-tumor effect. The utilization of this compound encounters various challenges results from releasing hydrogen cyanide. Apparently, the nano-formulation approach can increase its therapeutic effects. The β-cyclodextrin (β-CD) as a cyclic oligosaccharide is widely used in drug delivery. In this study, we aimed to nano-formulate the amygdalin by β-cyclodextrin in order to increase its effect on MCF-7 cell line. The synthesized β-CD-Amygdalin nanoparticle size, surface morphology, and chemical structure were determined by dynamic light scattering (DLS), Scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR), respectively. Also, we calculated the drug loading (DL), entrapment efficiency (EE), and drug releases. The MTT assay, wound-healing assay, real-time PCR, and flow cytometry assays were carried out to evaluate the β-CD-Amygdalin and amygdalin effects on cell viability, migration, expression of migration-related genes, and apoptosis, respectively. The results showed that the nanoparticle synthesized in the mean diameter of 54.94 nm with − 27.9 mV zeta potential, uniformity of shape, and expected structure. The DL and EE values were calculated in 17.5% and 90%. A slow curve of the amygdalin release profile with two burst release times in 6 h and 48 h was observed. The cellular and molecular evaluation of β-CD-Amygdalin and amygdalin effects on MCF-7 cells revealed that the β-CD-Amygdalin had greater effects than amygdalin. In conclusion, these results suggest that the nanoformulation of amygdalin with β-CD may elevate amygdalin therapeutic efficacy.

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Acknowledgement

This manuscript was supported by Tabriz University of Medical Sciences. Authors would like to thank Tabriz University of Medical Sciences for financial supporting of this project (Grant Number 61976).

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This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Bashir Mosayyebi, Mahsa Imani, Leila Mohammadi, Nosratollah Zarghami, Effat Alizadeh & Mohammad Rahmati

  2. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Bashir Mosayyebi

  3. Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Abolfazl Akbarzadeh

  4. Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Nosratollah Zarghami

  5. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Effat Alizadeh & Mohammad Rahmati

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  1. Bashir Mosayyebi
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Correspondence to Mohammad Rahmati.

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Mosayyebi, B., Imani, M., Mohammadi, L. et al. Comparison Between β-Cyclodextrin-Amygdalin Nanoparticle and Amygdalin Effects on Migration and Apoptosis of MCF-7 Breast Cancer Cell Line. J Clust Sci 33, 935–947 (2022). https://doi.org/10.1007/s10876-021-02019-2

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