In vitro Cytotoxicity and Antibacterial Activity of Optimized Silver Nanoparticles Against Wound Infectious Bacteria and Their Morphological Studies


The present study reports the optimized silver nanoparticles (AgNPs). They were tested against, three wound infecting gram-positive and gram-negative pathogenic bacteria viz., B. subtilis, S. aureus, M. luteus, E. coli, K. pneumoniae and P. aeruginosa at 10 µg concentration. In the antibacterial activity of optimized AgNPs, P. aeruginosa was found remarkably sensitive to the AgNPs with 20.3 ± 1.86 mm of inhibition zone. The standard antibiotics of streptomycin, gentamycin, ampicillin and erythromycin at 10 µg, when tested against the bacteria, which revealed that gentamycin showed high antibacterial activity against all the six wound infecting pathogenic bacteria. The MIC and MBC concentrations evaluated against the bacteria showed were 4.0 ± 1.00 µg/mL and 6.3 ± 0.47 µg/mL for S. aureus, respectively. The normal morphology of tested bacteria was changed when treated with optimized AgNPs as evidenced under TEM analysis. The optimized AgNPs impregnated on cotton fabrics showed high antibacterial activity against tested bacteria. Thereafter there was a gradual decrement in antibacterial activity against the bacteria at 2nd, 3rd and 4th wash. The cytotoxicity effect of optimized AgNPs treated normal cell (Vero) recorded a maximum IC50 value 20 µg/mL. Our research outcome opens up new improved antimicrobial composition in pharmaceutical and medical sectors.

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The authors would like to thank the Director, Centre for Advanced Studies in Botany, University of Madras, Chennai, and CSIR- Central Leather Research Institute (CLRI), Chennai, for providing the laboratory facilities. We thank NCN-SNT, University of Madras, for providing HRTEM, EDX and SAED analysis. We thank The Head, Centre for Nanoscience and Technology, Anna University for AFM analyses. We also thank the University Grants Commission-Bioscience Research (UGC-BSR) (No.F.4-1/2006/(BSR)5-61/2007(BSR) dt 29 Jun 2012.) herbal science scheme, New Delhi.

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Pannerselvam, B., Alagumuthu, T.S., Cinnaiyan, S.K. et al. In vitro Cytotoxicity and Antibacterial Activity of Optimized Silver Nanoparticles Against Wound Infectious Bacteria and Their Morphological Studies. J Clust Sci 32, 63–76 (2021).

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  • Optimized AgNPs
  • Wound infectious bacteria
  • Antibacterial activity
  • AgNPs coated fabric
  • Cytotoxicity