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Biofabrication of AgNPs Nanoparticles and Their Loading with Sevoflurane, a Volatile Anesthetic Drug for Therapeutic Treatment of Focal Cerebral Ischemia-In-Vivo Studies in Rats

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Abstract

The process of green reduction was used to prepare the colloidal mixture of silver nanoparticles (AgNPs) with the help of silver nitrate (AgNO3), which is used as a precursor along with the existence of Bischofia javanica leaf extract that plays both as a reducing as well as capping agent. An aesthetic drug which is volatile in nature and is used to form nano-drug complex (SF-AgNPs), Sevoflurane (SF) was used to functionalize the fabricated AgNPs. The interaction and loading of the drug with AgNPs is studied with the help of several analytical mechanisms such as dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and ultraviolet–visible (UV–Vis) absorption spectra. Several reflections in the XRD spectra and the shift observed in the SPR peak of UV–Vis is accredited to the loading of SF on the surface of AgNPs. When related with the bare NPs, the growth in the size of SF-loaded NPs confirms the loading of drug. Further investigation disclosed that the SF-AgNPs of 20 nm increased the fabrication of anti-inflammatory cytokines, along with the inhibition of ischemia–reperfusion induced microglias and astrocytes activation. In addition, the molecules of pro-apoptotic were down regulated and the expressions of anti-apoptotic proteins were up regulated in the post-ischemic brains. When examining the group treated with 15 nm AgNPs, these effects were noticed to be conflicting. These responses exposed that the neuroprotection of SF-AgNPs 20 nm was ascribable to its anti-apoptotic as well as anti-inflammatory effects. The present work can deliver a unique approach for the healing of cerebral ischemia–reperfusion injury. Owing to the above results, this work provided modernized understandings into the self-treatment of SF-AgNPs for cerebral ischemic stroke (IS).

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References

  1. M. G. Bousser (2012). Front Med.6, 22.

    Article  Google Scholar 

  2. C. J. Murray and A. D. Lopez (1997). Lancet.349, 1269.

    Article  CAS  Google Scholar 

  3. D. J. Cook, L. Teves, and M. Tymianski (2012). Nature.483, 213.

    Article  CAS  Google Scholar 

  4. J. P. MacManus, A. M. Buchan, I. E. Hill, I. Rasquinha, and E. Preston (1993). Neurosci. Lett.164, 89.

    Article  CAS  Google Scholar 

  5. T. Lelekov-Boissard, G. Chapuisat, J. P. Boissel, E. Grenier, and M. A. Dronne (2009). Transact. A Math. Phys. Eng. Sci.367, 4699.

    Article  CAS  Google Scholar 

  6. S. Wang, H. Wang, H. Guo, L. Kang, X. Gao, and L. Hu (2011). Neuroscience.185, 150.

    Article  CAS  Google Scholar 

  7. J. Jordan, T. Segura, D. Brea, M. F. Galindo, and J. Castillo (2008). Curr. Pharm. Des.14, 3549.

    Article  CAS  Google Scholar 

  8. S. B. Maddinedi, B. K. Mandal, and S. K. Maddili (2017). J. Photochem. Photobiol. B.167, 236.

    Article  CAS  Google Scholar 

  9. S. B. Maddinedi, B. K. Mandal, and K. K. Anna (2017). Environ. Toxicol. Pharmacol.49, 131.

    Article  CAS  Google Scholar 

  10. S. B. Maddinedi, B. K. Mandal, and K. K. Anna (2017). Environ. Toxicol. Pharmacol.51, 23.

    Article  CAS  Google Scholar 

  11. S. B. Maddinedi, B. K. Mandal, K. K. Anna, S. H. Patel, V. V. Andharkar, R. Shivendu, and D. Nandita (2017). J. Photochem. Photobiol. B.116, 252.

    Article  Google Scholar 

  12. R. F. Wallin, B. M. Regan, M. D. Napoli, and I. J. Stern (1975). Anesth. Analg.54, 758.

    Article  CAS  Google Scholar 

  13. T. J. Ebert, P. G. Schmid, P. G. Barash, B. F. Cullen and R. K. Stoelting (2001). III. Inhalation Anesthesia, Chapter 15, in Clinical Anesthesia, 4th ed. (Lippincott Williams & Wilkins: Philadelphia, PA, USA). p. 377.

  14. S. Chen and K. Kimura (2001). J. Phys. Chem. B.105, 5397.

    Article  CAS  Google Scholar 

  15. K. Anandalakshmi, J. Venugobal, and V. Ramasamy (2016). Appl. Nanosci.6, 399.

    Article  CAS  Google Scholar 

  16. K. Jeeva, M. Thiyagarajan, V. Elangovan, N. Geetha, and P. Venkatachalam (2014). Ind. Crops Products.52, 714.

    Article  CAS  Google Scholar 

  17. V. Kumar and S. K. Yadav (2009). J. Chem. Technol. Biotechnol.84, 151.

    Article  CAS  Google Scholar 

  18. X. Xiong, G. E. Barreto, L. Xu, Y. B. Ouyang, X. Xie, and R. G. Giffard (2011). Stroke.42, 2026.

    Article  Google Scholar 

  19. C. Benakis, C. Bonny, and L. Hirt (2010). Brain Behav. Immun.24, 800.

    Article  CAS  Google Scholar 

  20. J. Zaremba and J. Losy (2004). Neurol. Neurochir. Pol.38, S57.

    PubMed  Google Scholar 

  21. A. K. Abbas, K. M. Murphy, and A. Sher (1996). Nature.383, 787.

    Article  CAS  Google Scholar 

  22. J. Dokic, R. Rudolf, S. Tomic, S. Stopic, B. Friedrich, B. Budic, I. Anzel, and M. Colic (2012). J. Biomed. Nanotechnol.8, 528.

    Article  CAS  Google Scholar 

  23. M. P. Mattson, W. Duan, W. A. Pedersen, and C. Culmsee (2001). Apoptosis.6, 69.

    Article  CAS  Google Scholar 

  24. Y. Li, M. Chopp, N. Jiang, F. Yao, and C. Zaloga (1995). J. Cereb. Blood Flow Metab.15, 389.

    Article  CAS  Google Scholar 

  25. L. Li, L. Peng, and Z. Zuo (2008). Eur. J. Pharmacol.586, 106.

    Article  CAS  Google Scholar 

  26. P. H. Chan (2004). Research.29, 1943.

    CAS  Google Scholar 

  27. D. Tsuchiya, S. Hong, Y. Matsumori, T. Kayama, R. A. Swanson, W. H. Dillman, J. Liu, S. S. Panter, and P. R. Weinstein (2003). Neurosurgery.53, 1179.

    Article  Google Scholar 

  28. J. Y. Chan, H. L. Cheng, J. L. Chou, F. C. Li, K. Y. Dai, S. H. Chan, and A. Y. Chang (2007). J. Biol. Chem.282, 4585.

    Article  CAS  Google Scholar 

  29. V. Calabrese, C. Mancuso, A. Ravagna, M. Perluigi, C. Cini, C. De Marco, D. A. Butterfield, and A. M. Stella (2007). J. Neurochem.101, 709.

    Article  CAS  Google Scholar 

  30. A. Kader, V. I. Frazzini, R. A. Solomon, and R. R. Trifiletti (1993). Stroke.24, 1709.

    Article  CAS  Google Scholar 

  31. J. Cui, E. H. Holmes, T. G. Greene, and P. K. Liu (2000). FASEB J.14, 955.

    Article  CAS  Google Scholar 

  32. A. V. Dutra, H. F. Lin, S. H. Juo, H. Mohrenweiser, S. Sen, and R. P. Grewal (2006). BMC Med. Genet.7, 78.

    Article  Google Scholar 

  33. A. M. Alkilany and C. J. Murphy (2010). J. Nanopart. Res.12, 2313.

    Article  CAS  Google Scholar 

  34. N. Lewinski, V. Colvin, and R. Drezek (2008). Small.4, 26.

    Article  CAS  Google Scholar 

  35. N. Khlebtsov and L. Dykman (2011). Chem. Soc. Rev.40, 1647.

    Article  CAS  Google Scholar 

  36. S. Maiti (2011). J. Biomed. Nanotechnol.7, 65.

    Article  CAS  Google Scholar 

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Acknowledgements

Authors are thankful to Tai’an City central hospital, Shandong province for providing platform to do this research.

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Authors and Affiliations

  1. Department of Anaesthesiology, Luoyang Center Hospital Affiliated to Zhengzhou University, Luoyang, 471009, Henan, China

    Biao Zhou

  2. Department of Anesthesiology, Tai’an City Second Hospital of Traditional Chinese Medicine, Tai’an, 271000, Shandong, China

    Bo Cui

  3. Department of Anaesthesiology, Tai’an City Central Hospital, NO.29 Longtan Road, Tai’an, 271000, Shandong, China

    Wei Jing

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  1. Biao Zhou
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Correspondence to Wei Jing.

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Zhou, B., Cui, B. & Jing, W. Biofabrication of AgNPs Nanoparticles and Their Loading with Sevoflurane, a Volatile Anesthetic Drug for Therapeutic Treatment of Focal Cerebral Ischemia-In-Vivo Studies in Rats. J Clust Sci 31, 409–419 (2020). https://doi.org/10.1007/s10876-019-01654-0

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  • DOI: https://doi.org/10.1007/s10876-019-01654-0

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