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A Novel Design and Fabrication of Ascorbic Acid Sensitive Biosensor Based on Combination of HAP/rGO/AuNPs Composite and Ascorbate Oxidase

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Abstract

In this study, a new HAP/rGO/AuNPs-AO modified carbon electrode (GCE) was fabricated for amperometric detection of ascorbic acid. The HAP/rGO/AuNPs composite was prepared by one-step hydrothermal method. The large specific surface area, strong adsorption and excellent biocompatibility of HAP nanowires enabled fast, stable and accurate detection of ascorbic acid. As array of quantum dot, AuNPs had strong electrocatalytic activity in addition to good adsorption to enzymes, which could provide high sensitivity and low limit of detection for biosensor. Then rGO ensured that current signal could be quickly transferred from the composite to electrode. Under the optimum conditions, the prepared biosensor demonstrated a wide linear range from 0.39 to 36 mM (R2 = 0.99845), a low limit of detection (3.39 μM, S/N = 3) and a high sensitivity (15.949 mA moL−1) with good selectivity, stability (10.8%) and repeatability (3.9%), which might providing a reasonable and reliable way for ascorbic acid detection.

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Acknowledgements

The work is financially supported by the Fundamental Research Funds of Zhejiang Sci-Tech University (2019Y006) and the Science and Technology Program of Zhejiang Province of China (2017C31034).

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Authors and Affiliations

  1. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Chenyu Zhao, Wencui Zhou, Yong Zhang, Han Liu, Xiaogang Yang & Yurong Cai

  2. College of Foreign Languages, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Junfeng Jiao

  3. Department of Bioinformatics, Alagappa University, Karaikudi, Tamilnadu, 630003, India

    Boomi Pandi

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  1. Chenyu Zhao
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  2. Junfeng Jiao
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Zhao, C., Jiao, J., Zhou, W. et al. A Novel Design and Fabrication of Ascorbic Acid Sensitive Biosensor Based on Combination of HAP/rGO/AuNPs Composite and Ascorbate Oxidase. J Clust Sci 31, 337–346 (2020). https://doi.org/10.1007/s10876-019-01647-z

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  • DOI: https://doi.org/10.1007/s10876-019-01647-z

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