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Evaluation of Antibacterial and Anticancer Potential of Polyaniline-Bimetal Nanocomposites Synthesized from Chemical Reduction Method

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Abstract

This study gold (Au) and gold–platinum (Au–Pt) colloids were synthesized by borohydride reduction method in the presence of poly(N-vinyl-2-pyrrolidone) as a stabilizing agent. Besides, pristine polyaniline (PANI), PANI-Au nanocomposites, and PANI-Au–Pt nanocomposites were prepared. The synthesized nano-composites characterized by UV–Vis, FT-IR, XRD, and HR-TEM with energy dispersive X-ray techniques. Au and Au–Pt NPs were spherical in shape with particle sizes at 8 and 3 nm, respectively. PANI-Au and PANI-Au–Pt nanocomposites were also spherical in shape with particle sizes at 3 and 2 nm, respectively. Pristine PANI, PANI-Au, and PANI-Au–Pt nanocomposites were subjected to antibacterial activity against gram-positive (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Vibrio cholerae) bacterial pathogens. PANI-Au–Pt nanocomposite showed significant antibacterial activity (33 ± 1.10 mm dia) against B. subtilis. Besides, the MIC of pristine PANI, PANI-Au, and PANI-Au–Pt nanocomposites were found to be 75, 50 and 25 µg/mL, respectively. Besides, the in vitro anticancer investigations against HepG2 liver cancer cells revealed the highest cytotoxicity for PANI-Au–Pt nanocomposite (21.25 µg/mL) followed by PANI-Au nanocomposite (32 µg/mL) and pristine PANI (49 µg/mL). Overall, the present study showed considerable antibacterial and anticancer activity and suggested their potential use in pharmaceuticals after completing successful clinical trials for safety and affordability.

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Acknowledgments

The authors thank University Grants Commission, New Delhi for providing Assistant professor (No. F. 14-13/2013 (Inno/ASIST) dated 30.03.2013) under the Innovative scheme to carry out the teaching and research work. The authors thankfully acknowledge the DST-FIST [SR/FST/LSI-667/2016(C)], DST-PURSE [SR/PURSE Phase 2/38 (G)] and MHRD-RUSA 2.0 [F. 24/51/2014-U, Policy (TNMulti-Gen), Dept. of Edn. Govt. of India] for the financial supports and infrastructure facilities. The authors (H. Gurumallesh Prabu) acknowledge the UGC-BSR, New Delhi, India for the financial assistance by the onetime grant scheme.

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Authors and Affiliations

  1. Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India

    Pandi Boomi & Jeyaraman Jeyakanthan

  2. Kumaraguru College of Technology, Coimbatore, Tamil Nadu, 641049, India

    Gurumallesh Prabu Poorani

  3. Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, India

    Subramaniyan Palanisamy

  4. Department of Chemistry and Biochemical, Dongguk University College of Engineering, Jung-gu, Seoul, 04620, South Korea

    Samayanan Selvam

  5. PG. Department of Microbiology, Sri Paramakalyani College, Alwarkurichi, Tamil Nadu, India

    Ganesan Ramanathan

  6. Department of Biomedical Sciences, Alagappa University, Karaikudi, Tamil Nadu, India

    Sundaram Ravikumar

  7. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hamed Barabadi

  8. Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi, Tamil Nadu, India

    Halliah Gurumallesh Prabu

  9. Department of Medical Microbiology and Immunology, Institute of Biomedical Science, College of Health Sciences, Mekelle University, Mekelle, Ethiopia

    Muthupandian Saravanan

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  1. Pandi Boomi
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Boomi, P., Poorani, G.P., Palanisamy, S. et al. Evaluation of Antibacterial and Anticancer Potential of Polyaniline-Bimetal Nanocomposites Synthesized from Chemical Reduction Method. J Clust Sci 30, 715–726 (2019). https://doi.org/10.1007/s10876-019-01530-x

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